人类疱疹病毒6A亚型潜伏感染人神经胶质瘤细胞系U251  

Human herpesvirus 6A subtype latent infection of human glioma cell line U251

作　　者：顾斌[1] 张国锋[2] 李凌云[3] 周鋒 冯东举[4] 姚堃[4] 

机构地区：[1]东南大学附属中大医院神经外科,江苏南京210009 [2]九江市第一人民医院神经外科,江西九江332000 [3]南京医科大学发育遗传学系,江苏南京210029 [4]南京医科大学免疫学系,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2015年第10期1339-1343,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助(813016898;81273235;81201520);江苏省卫生厅科技项目(Z201409)

摘　　要：目的 :采用人神经胶质瘤细胞系U251作为宿主建立HHV-6A亚型潜伏感染神经系统细胞的体外模型。方法:HHV-6A急性感染U251细胞后随细胞传代,通过观察细胞形态变化、病毒复制及检测病毒基因表达情况等确定其进入潜伏感染阶段。结果:HHV-6A在急性感染U251细胞后随传代数的增加,病毒基因拷贝量逐渐减低至稳定的低水平,而代表处于潜伏感染阶段的HHV-6A病毒U94基因的转录逐渐下降随后维持在稳定水平表达。结论:HHV-6A在体外感染U251后通过传代使病毒从急性感染期逐渐转入潜伏感染期。Objective：To establish the model of human herpesvirus（HHV）-6A subtype latent infection of human glioma cell line U251 in vitro. Methods： U251 cells infected by HHV-6A were cultured and passaged for model establishment. The cell morphology changing, virus replication and viral gene expression were detected to identify the latent infection stage. Results： With increase of HHV-6A infection of U251 cells algebra, the viral gene copy number was gradually decreased to a stable low level. The HHV-6A U94 gene, a representative of the latent stage of infection of the virus, was gradually decreased and then maintained at a stable level. Conclusion：HHV-6A was successfully transferred from the acute infection stage to the latent infection stage in U251 cells in vitro.

关 键 词：人类疱疹病毒6A亚型 潜伏感染 U251 

分 类 号：R373[医药卫生—病原生物学]