短期暴露于不同剂量细颗粒物对哮喘小鼠气道炎症的影响  

Effects of fine particulate matter by different dosages at short-term exposure on airway inflammation in asthmatic mice

作　　者：刘燕[1,2] 冯旰珠[1] 杜强[1] 杜兴冉[1] 晋小祥[2] 

机构地区：[1]南京医科大学第二附属医院呼吸科,江苏南京210011 [2]马鞍山市中心医院呼吸科,安徽马鞍山243000

出　　处：《南京医科大学学报（自然科学版）》2015年第10期1364-1368,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81470209);江苏省自然科学基金(BK20131436);南京医科大学科技发展基金(2014NJMU141)

摘　　要：目的 :观察短期暴露于不同剂量细颗粒物(fine particulate matter,PM2.5)对哮喘小鼠气道炎症的影响,并初步探索PM2.5影响哮喘小鼠气道炎症机制。方法:将40只Balb/c小鼠采用随机数字表法分为5组:对照组、卵清蛋白(ovalbumin,OVA)组、OVA+低剂量PM2.5(10μg)组、OVA+中剂量PM2.5(31.6μg)组和OVA+高剂量PM2.5(100μg)组。通过腹腔注射OVA致敏、雾化吸入OVA构建小鼠哮喘模型,第26、28、30天予以PM2.5滴鼻激发。第31天处死小鼠后观察HE染色观察各组小鼠肺组织病理变化、炎性细胞浸润情况,比较各组小鼠肺泡灌洗液(BALF)中细胞总数及分类计数;并通过ELISA方法检测各组小鼠肺泡灌洗液中IL-3、IL-14及血清Ig E水平。结果:OVA+低剂量PM2.5组小鼠BALF中细胞总数、分类计数百分比及IL-4、IL-13水平与OVA组相比均无统计学差异(P>0.05);而OVA+中剂量PM2.5组及OVA+高剂量PM2.5组较OVA组升高(P<0.05)。OVA+低剂量PM2.5组和OVA+中剂量PM2.5组血清Ig E与OVA组相比轻度升高,但无统计学差异(P>0.05);OVA+高剂量PM2.5组较OVA组升高(P<0.05)。结论:中剂量PM2.5(31.6μg)和高剂量PM2.5(100μg)可进一步加重哮喘小鼠气道炎症;而低剂量PM2.5(10μg)对哮喘小鼠气道炎症无显著影响。Objective：To observe the effects of fine particulate matter（PM2.5） by different dosages at short-term exposure on airway inflammation in asthmatic mice, and study the mechanisms of PM2.5 on airway inflammation in asthmatic mice. Methods： A total of 40 BALB / c mice were randomly assigned into 5 groups, including the PBS control group, the OVA group（asthma group）,the OVA PM2.5（10 μg） group, the OVA PM2.5（31.6 μg） group,and the OVA PM2.5（100 μg） group. Asthma model was reproduced by sensitization with intraperitoneal injection and inhalation of OVA. On day 26, 28 and 30, the mice were performed with PM2.5intranasal stimulation, and then sacrificed on day 31. We observed the pathological changes and inflammatory cell infiltration by hematoxylin-eosin（HE） staining of lung tissues of mice alveolar fluid（BALF） cells in each group. IL-4 / IL-13 and serum Ig E levels were examined by the method of ELISA. Results： Compared with the asthma group, the differences in cell count and levels of IL-4 / IL-13 were not statistically significant in the PM2.5（10 μg） / OVA group（P〉0.05）, while those in the PM2.5（31.6 μg） / OVA group and the PM2.5（100 μg） / OVA group were increased（P〉0.05）. The level of serum Ig E in the PM2.5（10 μg） / OVA group and the PM2.5（31.6μg） / OVA group was increased slightly compared with the OVA group, but there was no significant difference（P 〉0.05）. However,the level of serum Ig E in the PM2.5（100 μg） / OVA group was significantly increased than that of the OVA group（P〈 0.05）.Conclusion： The asthma airway inflammation may further aggravate in the PM2.5（31.6 μg） / OVA and PM2.5（100 μg） / OVA,and no significant effect was from the PM2.5（10 μg） / OVA.

关 键 词：哮喘 PM2.5 白细胞介素4 白细胞介素13 

分 类 号：R562.2[医药卫生—呼吸系统]