建立大鼠心肌梗死模型时如何选择结扎位点和控制心肌梗死面积  

How to determine the position of ligation and control infarct size in a established rat myocardial infarction model

作　　者：侯予龙[1] 郭伟[1] 杨志健[2] 赵建强[1] 

机构地区：[1]南京医科大学附属淮安第一医院胸心外科,江苏淮安223300 [2]南京医科大学第一附属医院心脏科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2015年第10期1469-1473,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省博士后科研资助计划(1201079C);南京医科大学科技发展基金重点项目(2011NJMU237)

摘　　要：目的 :研究不同结扎位点对大鼠心肌梗死面积的影响,寻找有效、准确建立心肌梗死模型的方法。方法 :实验组建模前通过直视解剖显露大鼠心脏冠状动脉前降支,明确肺动脉圆锥与左心耳右缘之间交点和心尖的假想连线为大鼠冠状动脉前降支走行标志,选择在此连线上左心室最高点为缝扎位点建立大鼠心肌梗死模型(n=58)。对照组按传统方法建立大鼠心梗模型(n=38)。建模后,观察大鼠心电图和超声心动图改变,对比2组大鼠建模成功率;氯化三苯四唑(TTC法)检测比较2组大鼠心肌梗死面积。结果:建模后心电图示:实验组建模成功率明显高于对照组(88.9%vs 65.5%,P<0.01)。TTC法检测结果示:实验组中心肌梗死面积在中等梗死面积范围内的大鼠只数明显高于对照组(40只vs 11只,P<0.01)。结论:直视下显露大鼠心脏冠状动脉前降支,有助于准确识别冠状动脉前降支结扎位点,控制心肌梗死面积,提高建模成功率。Objective：The aim of the study was to determine the position of ligation and control infarct size in the establishing of a rat myocardial infarction model（MI）. Methods：Experimental group models（n =58） were produced by the ligation of left anterior descending coronary artery in left ventricular peak point along a line connecting the insertion of the left auricular appendage with the apex of the heart. MI control group（n =38） was produced by using the conventional method. Triphenyltetrazolium chloride（TTC）staining was performed to compare infarct size（IS,%） in two groups,and electrocardiogram（ECG） and echocardiography changes were observed also. Results：ECG monitoring showed that the success rate of the experimental group was significantly higher than that of the control group（88.9% vs 65.5%,P 〈0.05）. Moreover,there were 40 rats had medium-size infarction among experimental group detected by TTC. In contrast,only 11 rats had medium-size infarction among control group. It did differ significantly among rats with medium MI（P 〈0.01）. Conclusion：Accurately identify anterior descending coronary artery ligation site could help control the myocardial infarct size and improve the success rate of establishing myocardial infarction model.

关 键 词：心肌梗死面积 左冠状动脉前降支 氯化三苯四唑 多普勒超声心动图 

分 类 号：R654.2[医药卫生—外科学]