紫菜腐霉侵染对条斑紫菜叶状体防御性物质含量及保护性酶类活性的影响  

The Variation of Defensive Compounds Content and Protective Enzymes Activity of Pyropia yezoensis Gametophyte Infected by Oomycete Pythium porphyrae

作　　者：张晓南[1,2] 孙佩佩[1,2] 茅云翔[1,2] 邹丹丹[1,2] 唐祥海[1,2] 莫照兰[3] 

机构地区：[1]中国海洋大学海洋生物遗传学与育种教育部重点实验室,山东青岛266003 [2]中国海洋大学海洋生命学院,山东青岛266003 [3]农业部渔业资源可持续发展重点实验室中国水产科学研究院黄海水产研究所,山东青岛266071

出　　处：《中国海洋大学学报（自然科学版）》2015年第12期57-64,共8页Periodical of Ocean University of China

基　　金：国家自然科学基金项目(31372517);国家高技术研究计划项目(2012AA10A406);山东省自主创新专项(2013CXC80202);国家科技基础条件平台-水产种质资源平台运行服务项目资助

摘　　要：研究了紫菜腐霉侵染对条斑紫菜叶状体防御性物质脯氨酸(Pro),保护性酶类苯丙氨酸转氨酶(PAL)、多酚氧化酶(PPO),防御信号活性氧(ROS)和细胞损伤指示性物质丙二醛(MDA),在不同时间点(0、1、3、5、7、9d)的含量和活性影响。实验结果表明:紫菜腐霉侵染能够打破细胞ROS的动态平衡,引起ROS含量极显著增加(P<0.01),进而影响细胞渗透压平衡,表现为Pro含量极显著增加(P<0.01),并导致细胞内保护酶类PAL和PPO活性极显著增加(P<0.01),并且ROS积累引起细胞膜脂过氧化,MDA含量发生极显著变化(P<0.01)。由此推测条斑紫菜叶状体对紫菜腐霉侵染的生理响应过程为:紫菜腐霉侵染引起藻体ROS含量积累上升,积累的ROS激活机体防御系统,Pro含量增加以调节细胞渗透平衡;同时积累的ROS激活细胞内保护酶类,PAL和PPO的活性增强。迅速积累的ROS引起细胞损伤,导致细胞膜脂过氧化,产生并积累MDA。本实验为全面了解条斑紫菜防御紫菜腐霉侵染的机制奠定了基础,同时为后续条斑紫菜赤腐病抗性品系的选育提供了理论指导。In this study,the content of defensive compounds （ROS,Pro）,the activity of the protective enzymes （PAL,PPO） and MDA in Pyropia yezoensis gametophyte during the course of infection by Pythium porphyrae at different times （0,1,3,5,7,9 days） were measured.The result indicated that the infection of Pyt.Porphyrae could break the dynamic balance of ROS in cells that leads to its significant increase（P＜0.01）,and the osmotic balance was broken.Pro content was raised markedly（P＜0.01）,and the activity of protective enzymes such as PAL,PPO was found to be enhanced appreciably（P ＜0.01）,as a result the cells membrane was damaged,and MDA content was changed.Based on these data,we inferred an outline that defensive compounds and protective enzymes of Pyr.yeozensis respond to Pyt.porphyrae infection as follows.：the content of ROS in cells of Pyr.yeozensis would increase while induced by the infection of the pathogen.When the host cells received much ROS,the content of Pro was increased and the activity of PAL and PPO was enhanced indicating that the defensive system and the activity of the protective enzymes were activated.At last the cell membrane of the host were damaged by the excess ROS,leading to the lipid peroxidation of the cells membrane and high MDA levels.The results provided the fundamental data for understanding the mechanism of red rot disease resistance in P.yezoensis.

关 键 词：条斑紫菜 紫菜腐霉 侵染 防御性物质 保护酶类 丙二醛 

分 类 号：Q946.5[生物学—植物学]