黄蜀葵茎枯病病原菌的分离与鉴定  

Isolation and identification of pathogen causing stem blight disease on Abelmoschus manihot( L. ) Medic

作　　者：夏敏媛[1] 张瑜[1] 沈小林[2] 秦军[2] 缪轶君 谈献和[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏苏中药业集团股份有限公司,江苏姜堰225500

出　　处：《中国生化药物杂志》2015年第9期12-14,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：江苏高校优势学科南京中医药大学中药学学科开放研究课题(2011ZYX6-014)

摘　　要：目的分离黄蜀葵茎枯病病原菌并鉴定其种类。方法取江苏省宜兴市采集的黄蜀葵发病植株病灶处(J)和健康处(W)的茎段,培养并筛选新生菌丝体,将分离获得的几种待定病原菌分别接种至健康黄蜀葵幼苗茎部,进行致病性检测,随后将获得的有效致病菌株进行病原菌形态学鉴定、PCR扩增和r DNA-ITS检测以确定菌种。结果排除J与W中一致的菌种,分离得到3种真菌J2,J5和J6;致病性检测结果发现J5为有效致病菌;根据病原菌形态特征观察,初步鉴定J5为木贼镰刀菌;J5病原菌基因组DNA扩增后得到了长度为524 bp的条带,与木贼镰刀菌(Fusarium equiseti)的ITS序列同源性最高,达到了100%。结论黄蜀葵茎枯病病原菌为木贼镰刀菌F.equiseti。Objective To isolation and identify the pathogen of stem blight of Malvaceae. Methods The stems were collected from stem blightdiseased plants（ J） and healthy ones（ W） of Abelmoschus manihot（ L.） Medic. in Yixing City of Jiangsu Province then cultured to isolate newborn mycelium. The pathogen isolated but unidentified were inoculated in stems of healthy plants of Abelmoschus manihot（ L.） Medic. and pathogenicity was verified. Finally,the pathogenic specie（ s） was or were identified by morphological characteristic,r DNA-ITS analysis and polymerase chain reaction（ PCR） method. Results The same fungus were excluded which were the same species in J and W,the three fungus of J2,J5 and J6 were acquired. J5 was preliminarily identified to have pathogenicity and it was Fusarium equiseti under the microscope. The genome DNA of J5 was amplified to a length of524 bp,and homology highly with Fusarium equiseti（ 100%）. Conclusion The pathogen was identified as Fusarium equiseti.

关 键 词：黄蜀葵 茎枯病 致病菌 木贼镰刀菌 

分 类 号：S435.67[农业科学—农业昆虫与害虫防治]