SD大鼠富血小板血浆血小板聚集实验的改进  

Improvement Method of SD Rats Platelet-rich Plasma Platelet Aggregation

作　　者：沈道谦[1] 祝宁侠[1] 杨婧立 刘斌[1] 

机构地区：[1]汕头大学医学院心血管研究中心,广东汕头515041

出　　处：《汕头大学医学院学报》2015年第4期193-195,共3页Journal of Shantou University Medical College

基　　金：广东省自然科学基金资助项目(S2013010014519)

摘　　要：目的:探讨SD大鼠富血小板血浆(PRP)血小板聚集实验的改进方法。方法:采集成年SD大鼠血浆,对照组只加入花生四烯酸(AA,1.5 mmol/L)或二磷酸腺苷(ADP,10μmol/L),实验组分别加入不同浓度(1.0、2.5、3.5、5.0 mmol/L)Ca Cl_2,并分别加入AA或ADP。用血浆比浊法检测血小板聚集率。结果:对照组(AA、ADP)血小板聚集率分别为23.1±7.5、40.3±6.9,实验组以2.5 mmol/L Ca Cl_2时的血小板聚集率最高(44.5±5.5、80.1±5.4),且此浓度的Ca Cl_2不引起自身诱导的血小板聚集反应。结论:加入2.5 mmol/L Ca Cl_2可使SD大鼠PRP血小板聚集率最高。Objective：To investigate the improvement method of platelet-rich plasma（PRP）platelet aggregation. Methods：Plasma was derived from adult SD rats. The control group was added only arachidonic acid（AA,1.5 mmol/L）or adenosine diphosphate（ADP,10 μmol/L）,the experimental group was added different concentrations（1.0、2.5、3.5、5.0 mmol/L）of calcium chloride（Ca Cl2）,then added AA or ADP respectively. Turbidimetry was used to determine platelet aggregation. Results：The platelet aggregative rates（AA,ADP）in the control group were 23.1±7.53,40.3±6.9 respectively,44.5±5.5,80.1±5.4 in the experimental group when Ca Cl2 concentration of 2.5 mmol/L,and this concentration of Ca Cl2 alone derived platelet aggregating reaction was not observed. Conclusion：The method for SD rats PRP platelet aggregation is improved by adding 2.5 mmol/L Ca Cl2.

关 键 词：血小板 富血小板血浆 聚集 氯化钙 

分 类 号：R331[医药卫生—人体生理学]