白细胞介素6基因启动子-634C/G多态性与冠心病的关联  

Association between interleukin-6 promotor-634C / G polymorphism and coronary artery disease

作　　者：黄荣[1] 潘闽[1] 蔡星星[1] 顾周山[1] 苏亚民[1] 朱健华[1] 

机构地区：[1]南通大学附属医院心内科,江苏南通226001

出　　处：《南京医科大学学报（自然科学版）》2015年第11期1557-1560,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南通市临床医学专项资助(HS2013069)

摘　　要：目的 :探讨白细胞介素(interleukin t,IL)-6基因启动子-634C/G多态性与冠心病的相关性。方法 :纳入86例冠心病及91例对照组患者,检测相关生化指标,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测两组患者IL-6基因型,酶联免疫吸附(ELISA)法检测IL-6血清水平。比较两组间基因型、等位基因频率以及不同基因型对血清IL-6水平的影响。结果:冠心病组与对照组间年龄、性别、吸烟史、高血压病史及相关生化指标未见统计学差异(P>0.05),冠心病组外周血IL-6水平明显高于对照组[(26.57±6.68)ng/L vs.(6.89±2.32)ng/L,P<0.05],IL-6基因启动子-634C/G 3种基因型(CC、CG、GG)在冠心病组中的频率为57%、29%、14%,在对照组中的频率为65.9%、31.9%、2.2%,两组间基因型频率存在统计学差异(P<0.05)。冠心病组中IL-6基因启动子位点G等位基因携带者(GG、CG基因型)血清IL-6水平高于不携带者[(34.575±6.618)ng/L vs.(16.758±5.182)ng/L,P<0.05]。结论:IL-6基因启动子634C/G基因多态性与冠心病相关,其中G等位基因携带者外周血IL-6水平显著增高。Objective： To explore the association of-634 C / G polymorphism of interleukin-6（IL-6） gene with coronary artery disease（CAD）. Methods： A total of 86 CAD patients and 91 healthy controls were selected for this study. The PCR-based restriction fragment length polymorphism（PCR-RFLP） technique was performed to assess the genotypes frequencies. The concentration of serum IL-6 was measured with ELISA. The relations of the levels of serum IL-6 between groups were analyzed. Results： The circulating IL-6 levels in the CAD group[（26.57 ± 6.68） ng / L] were significantly higher than those in the control group [（6.89 ± 2.32） ng / L,P〈0.05]. The distribution of the IL-6-634 C / G genotypes（CC,CG, and GG） was 65.9%,3 1.9% and 2.2% in the controls,and 57%, 29%and 14% in the CAD group, respectively（P〈0.05）. Compared with the wild type CC,the G allele carriers（CG＋GG genotypes） had higher circulating IL-6 levels[（34.575 ± 6.618）ng / L vs.（16.758 ± 5.182）ng / L, P〈0.05] in the CAD group. Conclusion：These findings suggest that IL-6-634 C / G polymorphism is associated with CAD and the G allele carriers have increased circulating IL-6 levels.

关 键 词：白细胞介素6 冠心病 基因多态性 

分 类 号：R541.4[医药卫生—心血管疾病]