miR-203在系统性红斑狼疮患者外周血单个核细胞中的表达及临床意义  

Expression of miR-203 in peripheral blood mononuclear cells in patients with systemic lupus erythematosus

作　　者：王艳艳[1] 王嫱[1] 欧阳春[2] 

机构地区：[1]南京医科大学第一附属医院风湿免疫科,江苏南京210029 [2]南京医科大学第一附属医院肾内科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2015年第11期1594-1596,1623,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81302575);江苏省自然科学基金(BK20131028)

摘　　要：目的:探讨系统性红斑狼疮(systemic lupus erythematosus,SLE)患者外周血单个核细胞中mi R-203的表达水平与疾病活动度、肾脏受累情况等临床表现之间的关系,初步探讨mi R-203在SLE中的临床意义。方法:收集31例SLE患者、16例健康志愿者外周血标本,根据肾脏受累分为狼疮肾炎21例和SLE无肾脏受累10例。采用实时荧光定量聚合酶链反应(real-time fluorescence quota polymerase chain reaction,q RT-PCR)检测外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)中mi R-203表达,比较各组间mi R-203表达水平的差异,并分析其与SLE临床指标之间的关系。结果 :SLE患者mi R-203表达明显低于正常对照组,差异具有统计学意义(P<0.05);狼疮肾炎组患者mi R-203表达低于SLE无肾脏受累组和正常对照组,差异具有统计学意义(P<0.01);SLE患者mi R-203表达水平与24 h尿蛋白、肾脏急性指数呈明显负相关(P<0.01)。结论:SLE患者PBMCs中mi R-203的表达水平降低,表明mi R-203在SLE发病机制中可能发挥一定作用;狼疮肾炎患者mi R-203的表达降低,提示mi R-203可能参与了狼疮肾炎的发病。Objective：To explore the expression level of mi R-203 in the peripheral blood mononuclear cells（PBMCs） of patients with systemic lupus erythematosus（SLE）, and to analyze the relationship between the expression level and clinical manifestations.Methods： The PBMCs of 31 SLE patients, 16 healthy people were collected,and then SLE patients were divided into two groups,including the lupus nephritis group（21 cases） and the no kidney involvement group（10 cases）. The expression level of mi R-203 in PBMCs was detected by real-time fluorescence quota polymerase chain reaction（q RT-PCR）. The expression levels of mi R-203 in each group were compared to analyze the relationship between the expression level and clinical manifestations. Results： The expression level of mi R-203 was significantly lower in the SLE group compared to the control group（P〈0.05）. The expression level of mi R-203 was significantly lower in the lupus nephritis group than that in the no kidney involvement group and the control group（both P〈0.01）. The expression level of mi R-203 was negative correlated to 24 hours urine protein, acute kidney disease index（P〈0.01）. Conclusion： The expression of mi R-203 was decreased in SLE patients, indicating that mi R-203 may play a role in the pathogenesis of SLE. And mi R-203 may be associated with the occurrence of lupus nephritis.

关 键 词：系统性红斑狼疮 miR-203 外周血单个核细胞 

分 类 号：R593.24[医药卫生—内科学]