蟒蛇肉酶解肽电喷雾多级质谱法分析  

Python Enzymatic hydrolysis peptide by ESI-MSn

作　　者：高颖[1] 高晓晨[1] 李晶峰[1] 郭晓庆[1] 张辉[1] 孙佳明[1] 

机构地区：[1]长春中医药大学,长春130117

出　　处：《吉林中医药》2015年第12期1250-1251,1259,共3页Jilin Journal of Chinese Medicine

基　　金：吉林省教育厅"十二五"科学技术研究项目(吉教科合字[2012]第77号)

摘　　要：目的采用电喷雾多级质谱法(ESI-MSn)对蟒蛇肉酶解肽进行了分析鉴定,从而研究蟒蛇活性作用的物质基础。方法采用电喷雾质谱法,将蟒蛇肉酶解肽直接进样分析,得到了混合肽的一级质谱图及目标肽类成分的二级质谱图。结果利用质谱工作站所能提供的离子信息,共鉴定了两个具有代表性的酶解肽,分别是三肽(VN)和四肽(AAVN)。结论蟒蛇肉酶解肽以三肽和四肽为主,本研究为蟒蛇进一步合理开发应用奠定了基础。Objective To Python enzymatic hydrolysis peptides were analyzed and identified by electrospray ionization mass spectrometry（ ESI-MSn）. Thereby,the research on the material basis of python activity. Methods The electrospray ionization mass spectrometry,the python enzymatic hydrolysis peptide of direct injection analysis,mixed peptide level mass spectrometry figure and target peptide constituents of tandem mass spectra were obtained. Results A total of two representative enzymatic hydrolysis peptides were identified by using the ion information provided by mass spectrometry,respectively is tripeptide（ AVN） and tetrapeptide（ AAVN）. Conclusion The main enzymatic hydrolysis peptides of Python are tripeptide and tetrapeptide,this study lays a foundation for further rational development and application of python.

关 键 词：蟒蛇 酶解肽 电喷雾多级质谱法 分析鉴定 

分 类 号：R282.74[医药卫生—中药学]