24种精浆生化指标与精液常规参数的相关性研究  

Correlations of 24 biochemical markers in seminal plasma with routine semen parameters

作　　者：张红烨[1] 陆金春[2] 冯瑞祥[2] 

机构地区：[1]南京医科大学附属南京医院/南京市第一医院检验科,江苏南京210006 [2]武警江苏总队南京医院检验科,江苏南京210028

出　　处：《中华男科学杂志》2015年第12期1087-1092,共6页National Journal of Andrology

基　　金：南京市江宁区科技发展计划项目(2012Aa20)~~

摘　　要：目的:调查24种精浆生化指标与精液常规参数之间的相关性。方法:66例生育力低下男性的精液常规参数包括精液量、精子浓度、精子总数、精子活动率及前向运动精子百分率(PR)按WHO5标准进行分析,相应精浆中的24种生化指标在进行质控的基础上,使用全自动生化分析仪和电解质分析仪进行测定,同时24种精浆生化指标所测结果与各精液常规参数的相关性被分析。结果:精浆中蛋白水平包括总蛋白(TP)、白蛋白(Alb)和球蛋白(Glb)均与精子浓度呈显著正相关,且精浆白蛋白水平与精子总数亦呈显著正相关;精浆谷草转氨酶(AST)活性和乳酸脱氢酶(LDH)活性与精子浓度和精子总数均呈极显著正相关;精浆碱性磷酸酶(AKP)活性与精液量呈显著负相关,而与精子活动率呈显著正相关;精浆α羟丁酸脱氢酶(αHBDH)活性与精子浓度和精子总数呈极显著正相关,相关系数(r)达0.7以上;尿素氮(UN)与精液量呈显著负相关,而肌酐(Cr)与精液量、精子浓度和精子总数均呈显著负相关;精浆葡萄糖(Glu)水平与精子浓度和精子总数均呈显著负相关;精浆甘油三酯(TG)与精液量呈显著正相关,而与精子活动率呈显著负相关;精浆谷丙转氨酶(ALT)、γ谷氨酰转移酶(GGT)、腺苷脱氨酶(ADA)、尿酸(UA)、总胆固醇(TC)、肌酸激酶(CK)及超敏C反应蛋白(hs CRP)未见与上述精液参数相关。精浆钾、钠、氯、钙、镁、磷中只有精浆氯与精液量呈显著负相关,余均无显著相关性。结论:精浆中许多生化指标与精液常规参数均密切相关,提示这些生化组分可能在精子发生、成熟和生理代谢过程中起作用。Objective： To investigate the correlations of 24 biochemical markers in the seminal plasma with routine semen parameters. Methods： According to the WHO5 standards,we analyzed the routine semen parameters of 66 subfertile men,including the semen volume,sperm concentration,total sperm count,sperm motility,and the percentage of progressively motile sperm（ PR）.Based on the calibration and quality control measures and using an automatic biochemistry analyzer or electrolyte analyzer,we detected24 biochemical markers in the seminal plasma of the patients,including total protein（ TP）,albumin（ Alb）,globulin（ Glb）,uric acid（ UA）,alanine aminotransferase（ ALT）,aspartate aminotransferase（ AST）,alkaline phosphatase（ AKP）,γ-glutamyltransferase（ GGT）,lactate dehydrogenase（ LDH）,creatine kinase（ CK）,alpha hydroxybutyrate dehydrogenase（ αHBDH）,adenosine deaminase（ ADA）,glucose（ Glu）,triglyeride（ TG）,total cholesterol（ TC）,urea nitrogen（ UN）,creatinine（ Cr）,high-sensitive C-reactive protein（ hs CRP）,K^＋,Na^＋,Cl～-,Ca,Mg,and phosphorus（ P）. Then we analyzed the correlations of the 24 biochemical markers with routine semen parameters. Results： The levels of the TP,Alb,and Glb proteins in the seminal plasma were positively correlated with sperm concentration,so was that of Alb with the total sperm count,and the AST and LDH activities with sperm concentration and total sperm count. The AKP activity in the seminal plasma was correlated negatively with the semen volume,but positively with sperm motility. The αHBDH activity exhibited a positive correlation with both sperm concentration and total sperm count,with a coefficient of correlation（ r） above 0. 7. The UN level was correlated negatively with the semen volume,so was the Cr level with the semen volume,sperm concentration,and total sperm count,and the Glu level with sperm concentration and total sperm count. The TG level was correlated positively with the semen volume,but negatively wit

关 键 词：生化指标 精浆 精液参数 相关性 

分 类 号：R698.2[医药卫生—泌尿科学]