外源蔗糖对盐胁迫条件下甘草幼苗生长及有效成分含量的影响  

Effect of exogenous sucrose on growth and active ingredient content of licorice seedlings under salt stress conditions

作　　者：柳福智[1,2] 杨军[1] 

机构地区：[1]甘肃农业大学,甘肃兰州730070 [2]兰州大学化学化工学院,甘肃兰州730000

出　　处：《中国中药杂志》2015年第22期4384-4388,共5页China Journal of Chinese Materia Medica

基　　金：甘肃农业大学盛彤笙科技创新基金项目(GSAU-STS-1429)

摘　　要：以甘草幼苗为实验材料,研究了外源蔗糖对NaCl胁迫条件下甘草幼苗生长及有效成分含量的影响。结果表明,在盐胁迫条件下,添加一定浓度的外源蔗糖后,甘草幼苗的日相对生长率呈升高趋势,可明显的消弱这种胁迫效应,说明外源蔗糖具有缓解盐胁迫的效应。总黄酮含量和苯丙氨酸解氨酶(PAL)活性呈极显著升高,外源蔗糖对盐胁迫下甘草幼苗根系生长的缓解作用在很大程度上与PAL活性的增强所引起的黄酮含量的增加有关,当外源蔗糖的浓度为10 mmol·L^(-1)时,PAL的活性达到最大,当外源蔗糖的浓度为15 mmol·L^(-1)时,PAL的活性又成下降趋势,表明这种缓解作用具有浓度效应。在施加不同浓度的外源蔗糖后甘草苷含量的变化跟总黄酮含量的变化相似,施加不同浓度的外源蔗糖后甘草酸含量比在盐胁迫下高,达到未受盐胁迫时的水平,外源蔗糖的浓度梯度对甘草酸积累的影响不明显。Licorice seedlings were taken as experimental materials,an experiment was conducted to study the effects of exogenous sucrose on growth and active ingredient content of licorice seedlings under NaCl stress conditions. The results of this study showed that under salt stress conditions,after adding a certain concentration of exogenous sucrose,the licorice seedlings day of relative growth rate was increasing,and this stress can be a significant weakening effect,indicating that exogenous sucrose salt stress-relieving effect. The total flavonoids and phenylalanine ammonia lyase（ PAL） activity were significantly increased,the exogenous sucrose can mitigated the seedling roots under salt stress,the licorice flavonoid content in the enhanced growth was largely due to the activity of PAL an increased,when the concentration of exogenous sucrose wae 10 mmol·L^（-1）,PAL activity reaching a maximum,when the concentration of exogenous sucrose was 15 mmol·L^（-1）,PAL activity turned into a downward trend,the results indicating that this mitigation has concentration effect. After applying different concentrations of exogenous sugar,the contents of liquiritin changes with the change of flavonoids content was similar. After applying different concentrations of exogenous sucrose,the content of licorice acid under salt stress was higher than the levels were not reached during salt stress,the impact of exogenous sucrose concentration gradient of licorice acid accumulation was not obvious.

关 键 词：外源蔗糖 盐胁迫 甘草 有效成分 

分 类 号：S567.71[农业科学—中草药栽培]