胆木叶和胆木的相似性研究  

Similarity between leaves of Nauclea officinalis and stems of Nauclea officinalis

作　　者：廉源沛[1] 谢达温[1] 原少伟[1] 李艳静[1] 丁岗[1] 黄文哲[1] 萧伟[1] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001

出　　处：《中国中药杂志》2015年第22期4433-4441,共9页China Journal of Chinese Materia Medica

基　　金：现代中药创新集群与数字制药技术平台项目(2013ZX09402203)

摘　　要：建立HPLC定量指纹图谱检测方法,对3批胆木叶和胆木进行测定,比较其异长春花苷内酰胺的含量和指纹图谱的差异,并采用LC-MS对指纹图谱色谱峰进行指认,获得胆木叶和胆木化学成分差异信息。胆木中的异长春花苷内酰胺的含量高于胆木叶;胆木叶和胆木指纹图谱色谱峰有差别,胆木叶的化学成分较胆木丰富;LC-MS检测结果表明胆木叶和胆木中主要化学成分为生物碱,胆木叶和胆木有7种相同的化学成分。胆木叶和胆木的相似性研究为开发胆木叶潜在药用价值和胆木资源的可持续利用提供一定的科学依据。The study is to develop a method to determine 3 batches leaves of Nauclea officinalis and stems of N. officinalis by HPLC. The differences between strictosamide contents and fingerprints was compared,then chromatographic peak of fingerprints was validated with the assistance of LC-MS. The strictosamide contents in stems of N. officinalis were higher than leaves of N. officinalis.The main chemical composition in leaves of N. officinalis and stems of N. officinalis were alkaloid which revealed by LC-MS. There are7 chemical compositions were same between them,but the chemical composition in leaves of N. officinalis is more than stems of N. officinalis. This provides a scientific basis for the development of the potential medicinal value of leaves of N. officinalis and the sustainable utilization of N. officinalis.

关 键 词：胆木叶 胆木 指纹图谱 LC-MS 

分 类 号：R284.1[医药卫生—中药学]