四逆汤中干姜与其他药味配伍后姜辣素成分的变化规律  

Change Law of Gingerols from Zingiberis Rhizoma in Sini Tang Before and After Compatibility with Other Herbs

作　　者：蒋燕萍[1] 彭伟[1] 曹丽梅[1] 游宇[1] 高飞[2] 傅超美[1] 

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室四川省中药资源系统研究与开发利用重点实验室--省部共建国家重点实验室培育基地,成都611137 [2]香港大学李嘉诚医学院中医药学院,香港999077

出　　处：《中国实验方剂学杂志》2015年第24期1-4,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81373987)

摘　　要：目的：探索四逆汤中干姜与其他药味配伍后,姜辣素成分6-姜辣素,8-姜辣素和6-姜烯酚含量的变化规律。方法：以四逆汤、干姜单煎液、干姜-附子合煎液、干姜-甘草合煎液为供试品,采用HPLC测定供试品中3种姜辣素成分的含量,流动相0.1%冰乙酸（A）-乙腈（B）梯度洗脱（0~30 min,60%~10%A;30~35 min,10%~60%A）,检测波长275 nm。结果：干姜配伍不同药味后,3种姜辣素成分含量均升高,依次为干姜-甘草合煎液〉四逆汤〉干姜-附子合煎液〉干姜单煎液。结论：干姜配伍附子后干姜中姜辣素成分含量升高,可为干姜可助附子回阳救逆提供参考;干姜配伍甘草后干姜中姜辣素成分含量大幅度升高,为甘草配伍干姜提升温补阳气之力提供了一定实验数据;干姜与附子、甘草共同配伍后干姜中姜辣素成分含量明显升高,说明四逆汤方中3味药相互依托、相互制约以共奏回阳救逆之效,体现了四逆汤组方的科学内涵。Objective： To investigate change law of contents of gingerols（ 6-gingerol,8-gingerol and 6-shogaol） from Zingiberis Rhizoma in Sini Tang before and after compatibility with other herbs in this prescription.Method： Taking Sini Tang,single decoction of Zingiberis Rhizoma,Zingiberis Rhizoma-Aconiti Lateralis Radix Praeparata decoction and Zingiberis Rhizoma-Glycyrrhizae Radix et Rhizoma decoction as test samples,contents of these three gingerols in samples were measured by HPLC. Result： Contents of 6-gingerol,8-gingerol and 6-shogaol increased after Zingiberis Rhizoma compatibility with different medicines, sequence was Zingiberis Rhizoma-Glycyrrhizae Radix et Rhizoma decoction 〉 Sini Tang 〉 Zingiberis Rhizoma-Aconiti Lateralis Radix Praeparata decoction 〉 single decoction of Zingiberis Rhizoma. Conclusion： Contents of gingerols increase after Zingiberis Rhizoma compatibility with Aconiti Lateralis Radix Praeparata, which provides scientific basis for Zingiberis Rhizoma enhancing efficacy of Aconiti Lateralis Radix Praeparata. Contents of gingerols increase sharply after Zingiberis Rhizoma compatibility with Glycyrrhizae Radix et Rhizoma,which provides a certain scientific data for Glycyrrhizae Radix et Rhizoma promoting Wenbu Yangqi effect of Zingiberis Rhizoma. Contents of gingerols increase obviously in Sini Tang, it indicates that these three medicines in Sini Tang are interplaying to play efficacy,which reflecting scientific connotation of formula establishment of Sini Tang.

关 键 词：四逆汤 干姜 配伍 6-姜辣素 8-姜辣素 6-姜烯酚 

分 类 号：R283.6[医药卫生—中药学] R945[医药卫生—中医学]