3种种源铁皮石斛叶黄酮类成分HPLC特征图谱比较  

Comparison of HPLC Characteristic Spectrum of Flavonoids on Leaves of Dendrobium officinale from Three Provenance Sources

作　　者：黄月纯[1] 谢镇山 任晋[2] 叶家宏[1] 魏刚[2] 

机构地区：[1]广州中医药大学第一附属医院,广州510405 [2]广州中医药大学,广州510006

出　　处：《中国实验方剂学杂志》2015年第24期37-40,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省科技基础条件建设项目(2013B060400022)

摘　　要：目的:研究不同产地种源铁皮石斛叶黄酮类成分HPLC特征图谱,并初步比较丹霞地貌种源(广东、福建、浙江、江西)、浙江本地种、铁皮兰种(广西、云南)特征图谱的差异。方法:采用Kromasil 100-5 C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相甲醇-水(梯度洗脱),检测波长340 nm,柱温25℃,流速0.8 m L·min^(-1)。采用国家药典委员会"中药色谱指纹图谱相似度评价系统软件"(2004A版)生成共有模式。结果:3种种源的23批样品共标示出14个共有特征峰。其中,10批丹霞种源样品标示出12个共有特征峰(峰14为最强峰),样品相似度为0.880~0.996;6批浙江本地种源样品标示出13个共有特征峰(主要峰的相对峰面积较接近),样品相似度0.774~0.981;7批铁皮兰种源样品标示出9个共有特征峰(峰10,峰11相对峰面积较大),样品相似度0.922~0.974。丹霞种源对照图谱与浙江本地种对照图谱、铁皮兰种对照图谱的相似度分别为0.395,0.061,铁皮兰种对照图谱与浙江本地种对照图谱相似度仅为0.370。结论:该分析方法准确可靠,重复性好;相同种源铁皮石斛叶黄酮类成分群基本一致,显示铁皮石斛叶内在的稳定性;不同产地种源成分群组成及比例存在明显差异,亦为快速区分丹霞种源、浙江本地种、铁皮兰种区域特征提供了依据。Objective： To study the HPLC characteristic spectrum of flavonoids on leaves of Dendrobium officinale from different provenance sources and initially compare the characteristic spectrum among Danxia landform（ Guangdong,Fujian,Zhejiang and Jiangxi）,Tiepilan（ Guangxi,Yunnan） and native species from Zhejiang.Method： HPLC was used with Kromasil 100-5 C18 column（ 4. 6 mm × 250 mm,5 μm）; with methanol-water（ gradient elution） as a mobile phase,detection wavelength of 340 nm,column temperature of 25 ℃,and flow rate of 0. 8 m L·min-1. A common mode was generated with Traditional Chinese medicine（ TCM） chromatographic fingerprint similarity evaluation software system（ 2004 A version） by the State Pharmacopoeia Commission. Result：14 common peaks were marked in 23 batches of leaves of D. officinale. Among them,12 common peaks were marked in 10 batches of samples from Danxia landform and the similarity was 0. 880-0. 996. 13 common peaks were marked in 6 batches of samples from Zhejiang landform and the similarity was 0. 774-0. 981. 9 common peaks were marked in 7 batches of samples from Tiepilan landform（ relative peak area was larger for peak 10 and peak 11） and the similarity was 0. 922-0. 974. The similarities of Danxia landform with 6 batches from Zhejiang and 7 batches from Tiepilan were 0. 395 and 0. 061,respectively. The similarity of Tiepilan landform with 6 batches from Zhejiang was 0. 370 only. Conclusion： The method is accurate and reliable,and has a better reproducibility. The main flavonoids components of the same provenance sources are basically the same,indicating internal stability of D. officinale,but significant difference was present in components and ratio for different regions and provenance sources,providing a reference to quickly discriminate the provincial characteristics among Danxia landform,Tiepilan and native species from Zhejiang.

关 键 词：铁皮石斛叶 种群区域 丹霞铁皮种 浙江本地种 铁皮兰种 特征图谱 

分 类 号：R284.1[医药卫生—中药学]