葛花解酲方对肝癌细胞原位移植瘤小鼠端粒酶蛋白表达水平的影响  

Effect of Gehua Jiecheng Fang on Protein Expression Level of Telomerase in Orthotopic Allograft HCC Mice

作　　者：王镜辉[1] 唐东昕[1] 黄慧[1] 罗莉[1] 龙奉玺[2] 李军[2] 王定雪[1] 杨柱[2] 

机构地区：[1]贵阳中医学院第一附属医院,贵阳550002 [2]贵阳中医学院,贵阳550025

出　　处：《中国实验方剂学杂志》2015年第24期73-78,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81160417,81460697);贵州省研究生工作站计划项目(黔教研合JYSZ字[2014]018);贵州省科学技术厅-贵阳中医学院科学技术联合基金项目(黔科合LH字[2014]7336号)

摘　　要：目的:探讨葛花解酲方对肝癌小鼠端粒酶蛋白的影响。方法:Balb/C小鼠32只,除正常组8只外,其余制备供瘤体,建立H22小鼠肝癌细胞原位移植瘤模型,并随机分成3组,每组8只,分别为模型组、葛花解酲方低、高剂量组(20,40 g·kg^(-1))。给药组以中药水煎剂ig,正常组代以生理盐水,观察小鼠一般情况;体重、肝脏指数的变化;HE染色观察肝脏病理损伤的情况;ELISA检测血清丙氨酸氨基转移酶(ALT),天门冬氨酸氨基转移酶(AST),谷氨酰转移酶(GGT)的变化;Western blot检测肝脏端粒酶蛋白含量的变化。结果:与正常组比较,模型组体重明显降低,肝脏指数,ALT,AST,GGT水平及端粒酶蛋白水平明显升高(P<0.05);与模型组比较,葛花解酲方高、低剂量组小鼠体重明显升高,ALT,AST,GGT及端粒酶蛋白水平明显降低(P<0.05)。病理结果显示,模型组小鼠肝组织和细胞异型性明显,给药后小鼠肝脏病理损伤有所减轻。结论:葛花解酲方能减轻肝脏损伤,保护肝功能,通过抑制端粒酶蛋白表达,抑制其异常激活引起的细胞无限增殖,对小鼠肝癌原位移植瘤有一定的防治作用。Objective： To investigate the influence of Gehua Jiecheng Fang（ GJF） on the hepatic protein expression level of telomerase in mice. Method： The totally 32 Balb / C mice were randomly divided into the normal group（ normal saline）,the model group（ normal saline）,the low-and high-dose GJF groups（ 20,40g·kg-1） of 8 mice each. The orthotopic allograft hepatic cellular cancer（ HCC） model of H22 ascites tumor cells was established. The general observation including the general growth in mice,weight,liver index was conducted the pathological changes of the left hepatic lobe was observes by using HE staining. The changes of alanine aminotransferase（ ALT）,aspartate aminotransferase（ AST）,glutamyltransferase（ GGT） were detected by using ELISA. The protein expression level of telomerase in hepatic tissues was detected by using Western blot. Result：Compared with the normal group,the weight decreased,liver index and the levels of ALT,AST,GGT and protein expression of telomerase increased in the model group（ P 〈 0. 05）. Compared with the model group,the weight increased,liver index and the levels of ALT,AST,GGT and protein expression of telomerase decreased in the lowand high-dose GJF groups（ P 〈 0. 05）. Hepatic tissue and cellular heteromorphism were obvious in the model mice and the damages were reduced in the GJF groups. Conclusion： GJF has certain effect on HCC model by reducing the liver damage,protecting the liver function,regulating the protein expression level of telomerase in mice and restraining the cellular immortalization.

关 键 词：葛花解酲方 肝癌 原位移植瘤 肝功能 端粒酶蛋白 

分 类 号：R285.5[医药卫生—中药学]