护肝片对四氯化碳致大鼠慢性肝损伤肝阴虚证的保护作用  

Effect of Hugan Tablets on Chronic Hepatic Injury Induced by Carbon Tetrachloride in Rats Model of Yin-deficiency Syndrome of Liver

作　　者：梁增荣 龙梓 陈少锐[2] 钟婉华[1] 崔升淼[1] 

机构地区：[1]广东药学院中药学院,广州510006 [2]中山大学药学院,广州510006

出　　处：《中国实验方剂学杂志》2015年第24期137-141,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东药学院重点培养教师计划项目

摘　　要：目的:研究护肝片对四氯化碳(CCl4)致大鼠慢性肝损伤肝阴虚证的保护作用。方法:将42只Wistar大鼠分为5组:溶剂对照组(n=10)、模型组(n=8)、一贯煎组(相当于生药6.75 g·kg^(-1),n=8)、护肝片高、低剂量组(0.81,0.27 g·kg^(-1),n=8),除溶剂对照组外,其余各组均皮下注射40%CCl4花生油溶液,每次3 m L·kg^(-1),每周2次,连续5周,第6周改为每周注射1次,第5,6周同时灌胃给予温热中药(相当于生药18 g·kg^(-1)),每日1次,造模共6周,建立慢性肝损伤肝阴虚证的大鼠模型。第6周后开始给予相应的药物灌胃治疗,每日1次,连续2周至第8周末。末次给药后处死动物,采集大鼠血清和肝组织标本,检测血清丙氨酸转氨酶(ALT)、醛固酮(ALD)、白蛋白(ALB);肝组织匀浆超氧化物歧化酶(SOD)、丙二醛(MDA);肝组织羟脯氨酸(Hyp);HE染色法对肝组织病理学形态检查;Western blot方法检测肝组织中还原型烟酰胺腺嘌呤二核苷酸磷酸氧化酶(NADPH oxidase,NOX)NOX2的蛋白表达。结果:与溶剂对照组比较,模型组大鼠血清ALT,ALD和肝组织Hyp,MDA显著升高(P<0.01),血清ALB和肝组织SOD显著降低(P<0.01)。与模型组比较,护肝片高、低剂量组均能显著降低大鼠血清ALT水平和肝组织MDA,Hyp的含量(P<0.01,P<0.05);护肝片高剂量组显著降低血清ALD水平(P<0.05),升高血清中ALB含量(P<0.05)和肝组织中SOD活性(P<0.05),降低肝组织中NOX2的蛋白表达(P<0.05)。病理结果显示护肝片高、低剂量组明显减轻慢性肝损伤且一定程度降低纤维化水平。结论:护肝片能保肝降酶,对慢性肝损伤有明显的保护作用。Objective： To study the effect of Hugan tablets on chronic hepatic injury induced by carbon tetrachloride（ CCl4） in rat models of yin-deficiency syndrome of liver. Method： Forty-two Wistar rats were divided into five groups： dissolvent control group（ n = 10）,model group（ n = 8）,Yiguanjian decoction group（ equivalent to crude drugs 6. 75 g·kg-1,n = 8）,Hugan tablets high-dose group（ 0. 81 g·kg-1,n = 8） and lowdose group（ 0. 27 g·kg-1,n = 8）. Except the dissolvent group,all the other groups were injected with 40% CCl4 peanut oil solution,twice per week and 3 m L·kg-1each time,for 5 weeks,but one time per week in week 6. In week 5 and week 6,the rats were also orally taking interior warming drugs（ crude drugs 18 g·kg-1） once per day. After modeling for 6 weeks,rat models of yin-deficiency syndrome of liver with chronic hepatic injury was induced. From week 6,the rates received corresponding drug lavage treatment,once a day and for 2 to 8consecutive weeks. Rats were sacrificed after the last administration,and the serum and liver tissue were taken.Serum alanine aminotransferase（ ALT）,aldosterone（ ALD）,albumin（ ALB）,hydroxyproline（ Hyp） in liver tissues,malondialdehyde（ MDA）,and superoxide dismutase（ SOD） in liver tissues were determined. Pathological change of liver was determined and observed by hematoxylin-eosin staining. The protein expression of reduced from of nicotinamide-adenine dinucleotide phosphate（ NADPH） oxidase 2（ NOX2） in liver was analyzed by westernblot. Result： Compared to dissolvent group,the model group increased significantly the serum ALT,ALD as well as Hyp in liver tissues,significantly increased MDA（ P 〈 0. 01）,and significantly reduced the level of serum ALB and SOD in liver tissues（ P 〈 0. 01）. Compared to model group,the level of serum ALT,MDA and Hyp in liver tissue in Hugan tablets high-dose and low-dose group were significantly decreased（ P 〈 0. 01,P 〈0. 05）. Hugantablets high-dose group could sign

关 键 词：四氯化碳 温热中药 肝损伤 肝纤维化 护肝片 

分 类 号：R285.5[医药卫生—中药学]