番茄活体再生中反式玉米素含量的变化  

Change of Trans-zeatin in Tomato Regenerationin vivo

作　　者：曹慧颖[1] 张立军[1] 骆祯 刘玲莉[1] 孙建坤[1] 夏润玺[1] 

机构地区：[1]沈阳农业大学生物科学技术学院,辽宁沈阳110866

出　　处：《北方园艺》2015年第24期66-69,共4页Northern Horticulture

基　　金：国家自然科学基金资助项目(31300164);教育部博士学科点专项科研基金资助项目(20122103120004)

摘　　要：以番茄活体植株为试材,采用高效液相色谱(HPLC)法研究了番茄活体再生中反式玉米素含量的变化。结果表明:在愈伤组织的形成过程中,反式玉米素逐渐增加,并且在愈伤组织最发达时达到峰值。随着愈伤组织分化,其含量略有降低。此变化规律与番茄离体再生中外源反式玉米素使用浓度由高到低的变化规律基本吻合。活体再生中,用细胞分裂素合成抑制剂洛伐他丁处理伤口不影响再生进程。Taking tomato plant as material,change of trans-zeatin in vivo was researched by high performance liquid chromatography（HPLC）.The results showed that the content of trans-zeatin increased gradually with the formation of callus,and became peak in well-developed ones.When callus differentiated,the content decreased lightly.The content of trans-zeatin variation of regenerationin vivo was identical toin vitro.Lovastatin which could suppress the biosynthesis of cytokinins smeared in the cut surfaces could not impress the regeneration of tomato in vivo.

关 键 词：细胞分裂素 HPLC 再生机理 番茄 

分 类 号：S641.203.6[农业科学—蔬菜学]