蒲公英有机萃取物的抗甲型H1N1流感病毒作用  

Anti-influenza A(H1N1) Virus Effect of Organic Solvent Extracts from Taraxaci Herba

作　　者：王晓丹[1] 夏晓玲[2] 赵玉娇[1] 王新华[3] 张荣平[2] 孙强明[1] 

机构地区：[1]北京协和医学院/中国医学科学院医学生物学研究所、云南省重大传染病疫苗研发重点实验室,昆明650118 [2]昆明医科大学,昆明650500 [3]广州医科大学附属第一医院,广州医科大学呼吸疾病国家重点实验室,广州510230

出　　处：《中国现代应用药学》2015年第12期1423-1427,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金(81460593,81171946);云南省科技计划项目(2014FA031,2011CA016,2012FB188);中国医学科学院医学生物学研究所科技计划重点项目(2014IMB05ZD)

摘　　要：目的通过检测蒲公英不同的萃取物体外抗甲型H1N1流感病毒作用,探讨其在甲型H1N1流感治疗上的应用。方法首先用乙醇萃取而得到蒲公英的乙醇萃取物,然后依次采用乙酸乙酯、正丁醇、石油醚、水萃取蒲公英的乙醇萃取物,得到不同溶剂的萃取物;选用狗肾传代MDCK细胞作为病毒的宿主对病毒进行培养和扩增;通过血凝效价和Real time RT-PCR实验,间接或直接检测蒲公英不同萃取物对病毒的中和作用和增殖抑制作用。结果 4种不同溶剂的蒲公英萃取物作用病毒后,只有石油醚、乙酸乙酯组的血凝效价数值出现了一定程度的下降,其中乙酸乙酯萃取物组的值下降了2-4倍;而石油醚萃取物在96 h时,中和实验组和增殖抑制组的血凝效价值分别下降了256倍和128倍。Real time RT-PCR检测表明,石油醚和乙酸乙酯萃取物明显抑制病毒的扩增量。作用96 h时,无论是中和实验还是增殖抑制实验,石油醚萃取物的作用都是最显著的,病毒扩增率较阳性对照分别减少了92.4%和95.6%。结论蒲公英乙酸乙酯萃取物和石油醚萃取物在体外有明显的抗甲型H1N1流感病毒的作用。OBJECTIVE To investigate the anti-influenza A（H1N1） virus effect of organic solvent extracts from Taraxaci Herba by neutralization test and proliferation inhibition test in vitro. METHODS After Taraxaci Herba was extracted by alcoholate, the alcoholate extracts of Taraxaci Herba were extracted by ethyl acetate, n-butyl alcohol, petroleum ether and water in turn. Cytopathogenic effect（CPE） of MDCK cell infection by influenza A（H1N1） virus was observed with the interference of Taraxaci Herba extract based on cell culture technique. The anti-influenza A（H1N1） virus effect of neutralization test and proliferation inhibition test of Taraxaci Herba extracts were observed by the hemagglutination titre test and Real time RT-PCR. RESULTS Anti-influenza A（H1N1） virus neutralization and proliferation inhibition effect of four Taraxaci Herba extracts were observed after 96 h of treatment. The results of neutralization test showed that hemagglutination titer of ethyl acetate extracts were 2？4 times lower than the control group. While the results of neutralization and proliferation inhibition test showed that hemagglutination titer of petroleum ether extract was 256 and 128 times lower than the control group, respectively. According to the results of Real time RT-PCR test, the inhibition ratio of ethyl acetate extract and petroleum ether extract were lower than the control group. After 96 h of treatment by petroleum ether extract, amplification inhibition rate of neutralization and proliferation inhibition test were 92.4% and 95.6% lower comparing to the control, respectively. CONCLUSION Ethyl acetate and petroleum ether extract of Taraxaci Herba have anti-influenza A（H1N1） virus effects in vitro.

关 键 词：蒲公英 甲型流感病毒 狗肾传代细胞 抗病毒作用 萃取物 

分 类 号：R978.7[医药卫生—药品]