风铃玉的组织培养与快速繁殖  

Tissue Culture and Rapid Propagation of Ophthalmophyllum friedrichiae

作　　者：吴正景[1] 黄雪娇[1] 王柏[1] 彭涛 马文瑶[1] 

机构地区：[1]河南科技大学林学院,河南洛阳471003 [2]卢氏县中药材生产管理办公室,河南卢氏472200

出　　处：《植物生理学报》2015年第11期2013-2016,共4页Plant Physiology Journal

基　　金：河南科技大学博士科研基金(09001216)

摘　　要：以风铃玉叶片为外植体诱导愈伤组织并分化出不定芽发生,经生根得到再生植株,建立风铃玉离体再生体系。风铃玉愈伤组织诱导的较好培养基为MS+NAA 0.2 mg?L-1+6-BA 0.2 mg?L-1,诱导率为85.7%;最佳诱芽培养基是MS+IBA 0.02mg?L-1+6-BA 0.2 mg?L-1,能直接诱导出生长健壮的丛生芽;最佳生根培养基是1/2MS+IBA 0.1 mg?L-1,生根率高达83.3%。In this paper we studied in vitro culture of Ophthalmophyllum friedrichiae by using leaf as explants,including callus induction,adventitious shoot differentiation and rooting.The results indicated that MS＋NAA0.2 mg·L^（-1）＋6-BA 0.2 mg·L^（-1） was the suitable medium for callus induction,with an induction rate of 85.7%.The best medium for adventitious bud differentiation was MS＋IBA 0.02 mg·L^（-1）＋6-BA 0.2 mg·L^（-1）,and the shoots induced were strong.1/2MS＋IBA 0.02 mg·L^（-1）＋6-BA 0.2 mg·L^（-1） was the optimum medium for rooting,and the rooting rate reached 83.3%.

关 键 词：风铃玉 叶片 组织培养 快速繁殖 

分 类 号：S682.33[农业科学—观赏园艺]