食蟹猴体内自然成熟和体外培养成熟的卵母细胞对卵胞质内单精子显微注射(ICSI)结局的影响  

Impact of oocytes of Macaca fascicularis in vivo maturation and in vitro maturation on the outcome of intracytoplasmic sperm injection

作　　者：黄璋琼[1,2] 曾桥[3] 高家红[1,2] 江勤芳[1,2] 吴正存[1,2] 叶尤松 李聪[1,2] 李云[1,2] 马开利[1,2] 

机构地区：[1]中国医学科学院医学生物学研究所药物安全性评价研究中心,云南省重大传染病疫苗研发重点实验室,昆明650118 [2]中国医学科学院基础医学研究所,医学灵长类动物研究中心&神经科学中心,北京100005 [3]同济大学附属同济医院转化医学中心,上海200065

出　　处：《生殖与避孕》2015年第12期827-833,共7页Reproduction and Contraception

基　　金：国家自然科学基金(项目号:81301073,81571254)、国家自然科学基金-云南省联合基金(项目号:U1402221);高等学校博士点学科基金(批准号:20121106120056);云南省应用基础项目(项目号:2013FZ132,2011FZ211);协和青年基金–中央高校基本科研业务费专项资金(项目号:333 20140191);中国医学科学院病原生物学研究所基本科研业务费(项目号:2015IPB201)和中国医学科学院医学生物学研究所级一般项目(项目号:IMB2013YB01,2014IMB01ZD)资助的课题

摘　　要：目的：探讨食僖猴体内自然成熟和体外培养成熟（IVM）的卵母细胞对卵胞质内单精子显微注射（ICSI）结局的影响。方法：选取有正常月经周期和生育功能的13只健康成熟的雌性食蟹猴，使用优化的促排卵方案进行促排卵后，B超下观察用药后的卵巢情况，手术取卵后对已成熟的MⅡ期卵母细胞进行ICSI，未成熟的GV及MⅠ期卵母细胞在IVM16-38h后发育为MⅡ期卵母细胞，再进行ICSI，观察IVM及体内自然成熟的卵母细胞的受精情况和胚胎发育情况。结果：IVM为76．1％±14．9％，受精率为56．79％，卵裂率为73．91％，优质胚胎率为44．12％；体内自然成熟的卵母细胞受精率为68．52％，卵裂率为94．59％，优质胚胎率为58．57％。在受精率和卵裂率方面，两者间均有统计学差异（P〈0．05和P〈0．01），而优质胚胎率无统计学差异（P〉0.05）。结论：食蟹猴体外培养成熟的卵母细胞对ICSI结局有较大影响。Objective： To investigate the impact of oocytes of Macacafascicularis in vivo maturation and in vitro maturation （IVM） on the outcome of intracytoplasmic sperm injection （ICSI）. Methods： Thirteen healthy and sexual mature female cynomolgus monkeys were selected, ovarian ultrasound image was observed under ultrasound after ovulation induction through optimal protocol, then oocytes were taken by surgery, all mature M Ⅱ oocytes were performed with ICSI, immature oocytes （include GV and M1） were cultured in vitro, 16-38 h later, M Ⅱ oocytes of IVM were performed with ICSI, the fertilization of oocytes and the embryonic development in vitro culture were then observed and recorded. Results： The IVM rate of oocytes was 76.1%±14.9%, the fertilization rate, the cleavage rate and the high-quality embryo rate of M Ⅱ oocytes were respectively 56.79%, 73.91% and 44.12%. The fertilization rate, the cleavage rate and the high-quality embryo rate of IVM M Ⅱ ooeytes after in vivo maturation were respectively 68.52%, 94.59% and 58.57%. Compared between the two groups, there were obvious differences in the fertilization rate and the cleavage rate （P〈0.05 or P〈0.01）, but there was no obvious difference in high-quality embryo rate （P〉0.05）. Conclusion： Impact of IVM oocytes on the outcome of ICSI was obvious.

关 键 词：食蟹猴 卵母细胞 体内自然成熟 体外培养成熟(IVM) 卵胞质内单精子显微注射(ICSI) 

分 类 号：R714.8[医药卫生—妇产科学]