体外受精胚胎细胞数量和碎片比例对囊胚形成的影响  

Influence of cell number and fragment to blastula formation during in vitro fertilization-embryo transfer treatment

作　　者：廖桂芝[1] 丁洁[1] 顾斌[1] 郑爱燕[1] 蒲艳[1] 王玮[1] 

机构地区：[1]南京医科大学附属苏州市立医院,苏州215002

出　　处：《生殖与避孕》2015年第12期879-883,共5页Reproduction and Contraception

摘　　要：目的:探讨体外受精(IVF)周期中胚胎细胞数量和碎片比例对囊胚形成的影响。方法:回顾性分析2 404个IVF周期患者的13 037个进行囊胚培养的胚胎。根据第3日细胞数量将胚胎分为2~3-细胞组(2C^3C)、4-细胞组(4C)、5-细胞组(5C)、6-细胞组(6C)、7-细胞组(7C)、8-细胞组(8C)、9-细胞组(9C)、10-细胞组(10C)、11-细胞组(11C)、12-细胞组(12C)、≥13-细胞组(≥13C),分析囊胚培养细胞数量和碎片比例与囊胚形成的关系。结果:碎片为1~2级的胚胎,8C组和≥13C组囊胚形成率明显高于其他各组(P<0.05);7C、9C^12C组之间囊胚形成率无统计学差异(P>0.05)。碎片为3级的胚胎,8C组的囊胚形成率高于其他各组(P<0.05),且与碎片1~2级7C、9C^12C组的胚胎相比无统计学差异(P>0.05);9C组囊胚形成率高于7C组,且差异显著(P<0.05)。结论:第3日8C胚胎的发育潜能是最优的,可适当放宽对8C碎片的评估;随着碎片比例增大可减弱胚胎的发育潜能。Objective： To investigate the influence of cell number and fragment in day 3 embryo to blastula formation through blastocyst culture. Methods： Totally 13 037 embryos from 2 404 IVF treatment cycles were collected retrospectively. Blastocyst formation were observed till day 6 by blastocyst culture. According to the cell number in each embryo, the embryos were divided into 11 groups, which were 2-3-cell （2C-3C）, 4-cell （4C）, 5-cell （5C）, 6-cell （6C）, 7-cell （7C）, 8-cell （8C）, 9-cell （9C）, 10-cell （10C）, 11-cell （11C）, 12-cell （12C）, ≥ 13-cell （≥13C） groups. The relationship of blastocyst formation and fragment ratio to call number was analyzed. Results： When the fragment grade was below 2, the blastula formation rate in 8C group and ≥13C group was significantly higher than that in other groups （P〈0.05）; there were no differences among 7C and 9C-12C groups （P〉0.05）. When the fragment grade was 3, the blastula formatuion rate in 8C group was significantly higher than that in other groups （P〈0.05） and no significant differences were found among 7C and 9C-12C groups in fragment grade 2 （P〉0.05）; the blastula formation rate in 9C group was higher than that in 7C group （P〈0.05）. Conclusion： 8C embryo in day 3 had the best potentiality of development, and the fragment ratio can influence the development potential.

关 键 词：辅助生殖技术(ART) 体外受精(IVF) 囊胚培养 细胞数量 碎片比例 

分 类 号：R714.8[医药卫生—妇产科学]