精液分析区域性外部质量控制研究  

Regional external quality control for semen analysis

作　　者：王奇玲[1] 唐运革[1] 邓顺美[1] 庄嘉明[1] 李月华[1] 唐雨倩 陈曦[1] 马婷[1] 马春杰[1] 

机构地区：[1]国家卫生和计划生育委员会男性生殖与遗传重点实验室,广东省计划生育科学技术研究所人类精子库,广州510600

出　　处：《生殖与避孕》2015年第12期888-892,共5页Reproduction and Contraception

基　　金：广东省卫生计生委科技项(20132001)资助

摘　　要：目的:探讨精液分析区域性外部质量控制实践中参评实验室检测结果的准确性和结果的可比性。方法:制作2个水平的精子浓度、活动率和正常形态率的质控品发放给参评实验室进行外部质量控制测试,分析质控结果。评价指标为均值和实验室间变异系数(CV)。结果:精子浓度、前向运动精子百分率(PR)和正常形态精子百分率(NR)CV分别为34.09%、24.96%和62.01%;各实验室精子浓度、PR结果差异均无统计学意义(P>0.05),NR差异有统计学意义(P<0.05)。结论:外部质控结果CV较大,尤其正常形态精子的检测结果,提示技术人员亟需进行规范培训,开展标准化精液分析质量控制活动,提高分析结果的重复性和准确性。Objective： To explore the accuracy and comparability of results of the participating laboratories during regional external quality control （QC） for semen analysis. Methods： The QC samples were prepared and distributed to the participating laboratories for the external quality control testing. The mean value and the average coefficient of variation （CV） of sperm concentration, progressive motility rate （PR）, normal morphology rate （NR） were compared. Results： The average CV among laboratories for the sperm concentration, PR and NR were 34.09%, 24.96% and 62.01%, respectively. There was no significant difference for the sperm concentration, progressive motility among the laboratories （P〉0.05）, except for the normal morphology （P〈0.05）. Conclusion： The CVs for the three parameters were higher, especially for the normal morphology. There is an urgent need of regular standardized training for laboratory technicians to improve the results of semen analysis of repeatability and accuracy.

关 键 词：精液分析 区域性外部质量控制 平均变异值 变异系数(CV) 

分 类 号：R446.19[医药卫生—诊断学]