雷米普利与曲美他嗪对慢性心力衰竭大鼠肾脏细胞凋亡及细胞色素C的影响  

Effect of Ramipril and Trimetazidine on Renal Cell Apoptosis and Cytochrome C Expression in Experimental Rats With Chronic Heart Failure

作　　者：张梦云[1] 杨晓欧 彭玉娟[2] 冯翔宇 李秀华[1] 

机构地区：[1]承德医学院附属医院老年病科,河北省承德市067000 [2]泰安市第一人民医院心内科,山东省泰安市 [3]沧州市人民医院心内科,河北省

出　　处：《中国循环杂志》2015年第12期1208-1211,共4页Chinese Circulation Journal

基　　金：河北省卫生计生委资助项目(20110180)

摘　　要：目的:探讨雷米普利与曲美他嗪单用及联合应用对慢性心力衰竭(CHF)大鼠肾脏细胞凋亡及细胞色素C(Cyt-C)表达的影响。方法:采用肾上腹主动脉缩窄术复制CHF模型,将50只雄性Wistar大鼠随机分为假手术组、模型组、雷米普利组、曲美他嗪组和联合用药组(雷米普利+曲美他嗪),每组10只。利用TUNEL法检测肾小管上皮细胞凋亡;逆转录聚合酶链反应法检测Cyt-C m RNA含量以及Western Blot法检测肾脏细胞Cyt-C蛋白表达水平。结果:与假手术组比较,模型组肾小管细胞凋亡指数(AI)、Cyt-C m RNA含量及蛋白表达量均显著增加(P<0.01),与模型组比较,雷米普利组、曲美他嗪组和联合用药组的肾小管AI[(20.02±1.14)%、(20.10±1.20)%、(14.27±1.40)%vs(40.82±1.31)%]、Cyt-C m RNA表达量(0.54±0.06、0.56±0.05、0.44±0.04 vs 0.89±0.03)及蛋白表达水平显著降低(1.50±0.11、1.58±0.12、0.75±0.06 vs 2.53±0.10),且以联合用药组降低最明显,差异均有统计学意义(P<O.01)。结论:雷米普利与曲美他嗪均可抑制肾脏细胞凋亡,有效改善肾脏功能,联合用药对细胞凋亡的抑制作用优于单一用药。Objective： To explore the effects of ramipril, trimetazidine and the combination oframipril and trimetazidine on renal cell apoptosis index （AI） and cytochrome C （Cyt-C） expression in experimental rats with chronic heart failure （CHF）. Methods： CHF model was established by partially banding of abdominal aorta superior to renal artery in experimental rats. A total of 50 male Wistar rats were randomly divided into 5 groups： Sham operation group, Model group, Ramipril group, Trimetazidine group and Combination （ramipril and trimetazidine） group, n=10 in each group. Renal tubular cell AI was examined by TUNEL method, mRNA and protein expressions of Cyt-C were detected by RT-PCR and Western Blot analysis in each group respectively. Results： Compared with Sham operation group, Model group had increased AI of renal tubular ceils, increased mRNA and protein expressions of Cyt-C, P〈0.01. Compared with Model group, Ramipril group, Trimetazidine group and Combination group showed decreased AI of renal tubular cells （20.02±1.14） %, （20.10 ± 1.2） % and （14.27 ± 1.40） % vs （ 40.82 ± 1.31） %; reduced Cyt-C mRNA expression （0.54 ±0.06）, （ 0.56 ±0.05） and （0.44±0.04） vs （0.89± 0.03）; reduced Cyt-C protein expression （1.50± 0.11）, （1.58±0.12） and （0.75±0.06） vs （2.53±0.10）; the most reduction was obtain by Combination group, all P〈0.01. Conclusion： Ramipril and trimetazidine can inhibit renal cell apoptosis and effectively improve the renal function in CHF rats. Combined medication is better than either of them alone.

关 键 词：雷米普利 曲美他嗪 心力衰竭 细胞凋亡 

分 类 号：R541.6[医药卫生—心血管疾病]