山银花黄酮粗提物对MCF-7细胞增殖及凋亡的作用  

Antitumor Activities of Crude Extracts of Flavonoids from Flos Lonicerae Confusae on MCF-7 Cell

作　　者：谢雁飞[1] 徐望龙[2] 刘刚[1] 姚旭[1] 周斌[1] 郭玉[1] 

机构地区：[1]南华大学药物药理研究所,湖南衡阳421001 [2]娄底市中心医院药剂科,湖南娄底417000

出　　处：《肿瘤药学》2015年第6期420-424,共5页Anti-Tumor Pharmacy

基　　金：湖南省十二五重点学科建设项目资助(批准文号:湘教发(2011)76号);国家自然基金(项目批准号:81372579)

摘　　要：目的体外观察山银花黄酮粗提物对MCF-7细胞增殖和凋亡的影响,为其黄酮类化合物的进一步开发利用提供理论依据。方法 MTT法检测山银花粗提物对MCF-7细胞增殖的抑制作用;Hoechst33342染色法观察MCF-7细胞凋亡;流式细胞术检测MCF-7细胞的凋亡率。结果高于200μg·m L-1的山银花粗提物对MCF-7细胞增殖有显著抑制作用(P<0.05)。山银花粗提物对细胞形态有明显影响,随浓度的增加,细胞核被染成亮蓝色的细胞比例明显增加。0μg·m L-1、100μg·m L-1、300μg·m L-1、600μg·m L-1的山银花粗提物作用的MCF-7细胞凋亡率分别为(3.89±0.70)%、(10.40±0.92)%、(22.02±1.22)%、(27.03±0.75)%。结论山银花黄酮粗提物对MCF-7肿瘤细胞有明显的抑制增殖与诱导凋亡作用,其效应与浓度呈正相关。Objective To investigate the effects of flavoniods crude extract from Flos Lonicerae Confusae on the prolif- eration and apoptosis of MCF-7 cells, so as to provide the material foundation and scientific basis for further exploration and utilization. Methods The inhibition effects on proliferation of MCF-7 cells were tested by MTT. The morphological changes of MCF-7 apoptosis were observed by Hoechst 33342 staining. Flow cytometry was involved to measure the apoptosis rate of MCF-7 ceils. Results The flavoniods crude extract of concentration more than 200 μg·m L-1 had great inhibition effect on the proliferation of MCF-7 cells （P 〈 0.05 vs. control）. The crude extract also had a significant effect on cell morphology. The cell ratio of nucleus stained into hght blue increased along with the increase of concentration of the exlracts. The apoptosis rates of MCF-7 induced by a concentration of 100μg·m L-1, 300μg·m L-1 and 600μg·m L-1 were （10.4 ±0.92）%, （22.02 ±1.22）% and （27.03 ± 0.75）%, respectively. Conclusion The crude flavonoids extracts from Flos Lonicerae Confusae had great inhibition on proliferation and induction to apoptosis of MCF-7 cells. The effect was positively correlated with its concentration.

关 键 词：黄酮粗提物 抑制增殖 凋亡 

分 类 号：R965.2[医药卫生—药理学] R737.9[医药卫生—药学]