陆海渐渗系分离群体(F_2)产量和纤维品质性状的QTL定位  

Mapping QTL of Fiber Yield and Quality Traits in F_2 Populations of Chromosome Segment Substitution Lines from Gossypium hirsutum × Gossypium barbadense

作　　者：郭丽雪[1,2] 石玉真[2] 李俊文[2] 龚举武[2] 刘爱英[2] 商海红[2] 巩万奎[2] 陈婷婷[2] 葛群[2] 孙杰[1] 袁有禄[2] 

机构地区：[1]石河子大学农学院/新疆兵团绿洲生态农业重点实验室,新疆石河子832003 [2]中国农业科学院棉花研究所/棉花生物学国家重点实验室/农业部棉花生物学与遗传育种重点实验室,河南安阳455000

出　　处：《棉花学报》2015年第6期550-560,共11页Cotton Science

基　　金：国家自然科学基金(31101188);国家973计划项目(2010CB126006);国家高技术研究发展计划(863计划)项目(2012AA101108)

摘　　要：以优异的海岛棉染色体片段渐渗系MBI7455和MBI7358为亲本,构建F2分离群体。利用SSR（Simple sequence repeat）标记对亲本的基因型及F2群体产量和纤维品质的QTL（Quantitative trait locus）进行分析,结果表明母本MBI7455恢复到轮回亲本中棉所45背景的比例为96.70%,共渐渗12个海岛棉片段;父本MBI7358恢复到轮回亲本中棉所45背景的比例为95.60%,共渐渗16个海岛棉片段;F2群体平均背景恢复率为96.44%,渐渗海岛棉片段平均值为13.42个,纯合片段平均值为3.90个。QTL定位分析,在F2群体中共检测到26个QTL,其中与纤维品质相关的有19个,表型贡献率为2.52%~13.11%;与产量相关的有7个,表型贡献率为2.93%~11.40%。本研究利用染色体片段渐渗系定位与产量和纤维品质相关的QTL,为棉花分子标记辅助育种奠定了基础。In this study, F2 populations from two chromosome segment substitution lines MBI7455 and MBI7358, were quantified using SSR to evaluate the parents＇ genotype and detect QTL related to fiber quality plus yield traits of cotton. Results show that the recurrent parent（CCRI45） hosted 96.70% and 95.60% of chromosome segment substitution in MBI7455 with 12 chromosome segments and in MBI7358 with 16 chromosome substitution segments of Gossypium barbadense, respectively. In the F2 population, the average rate of chromosome substitution of the recurrent parent（CCRI45） was 96.44%, and the average segments of chromosome substitution of Gossypium barbadense was 13.42, with an average segments of homozygous donor chromosome value of 3.90. Analysis showed 19 fiber quality-related QTL with a phenotypic variance of between 2.52%-13.11% and seven yield traits-related QTL with a range of 2.93%-11.40% phenotypic variance, resulting in a total of 26 QTL. The CSSLs could be used to detect QTL for fiber yield and quality traits, which offer an important foundation for the cotton molecular-assisted breeding.

关 键 词：棉花 染色体片段渐渗系 QTL 纤维品质 

分 类 号：S562[农业科学—作物学]