激光扫描共聚焦显微镜检测分枝杆菌与溶酶体融合的实验教学设计  

Experimental teaching design on detection of fusion of mycobacterium and lysosome in macrophages by laser scanning confocal microscope

作　　者：乔可[1] 王晴岚[1] 牛辰[1] 

机构地区：[1]复旦大学基础医学院病原生物学系,教育部、卫生部医学分子病毒学重点实验室,上海200032

出　　处：《实验技术与管理》2015年第12期187-189,193,共4页Experimental Technology and Management

基　　金：国家自然科学基金青年项目(81201256)

摘　　要：介绍了使用经绿色荧光蛋白标记的海分枝杆菌侵染巨噬细胞,再利用激光扫描共聚焦显微技术检测分枝杆菌与巨噬细胞溶酶体融合的实验教学设计。该实验对研究分枝杆菌与巨噬细胞的相互作用以及分枝杆菌的致病机制具有重要意义,同时在教学实验中使学生更深入地理解激光扫描共聚焦显微镜检测技术,并培养学生的实践能力和科研创新意识。The experiment is designed in which macrophages were firstly infected by mycobacteria labelled with GFP marker,followed by detection of the fusion of mycobacteria and lysosomes in macrophages by laser scanning confocal microscope.This experiment is of great importance to the study of the interaction between mycobacteria and macrophages and the pathogenic mechanism of mycobacteria.Through this teaching experiment,students can get a deeper understanding on the laser scanning confocal microscope.Meanwhile,it can also cultivate the practical ability and innovation consciousness of the students in scientific research.

关 键 词：激光扫描共聚焦显微镜 绿色荧光蛋白 分枝杆菌 巨噬细胞 

分 类 号：G642.0[文化科学—高等教育学]