UPLC-Q-TOF/MS法鉴定大鼠体内甘草素的代谢产物  

Characterization of metabolites of liquirtigenin in rats by UPLC-Q-TOF/MS

作　　者：李元元[1,2] 江振作[1,2] 张蕾[1,2] 柴欣[1,2] 王跃飞[1,2] 

机构地区：[1]天津中医药大学天津市现代中药重点实验室,天津300193 [2]天津国际生物医药联合研究院中药新药研发中心,天津300457

出　　处：《天津中医药》2015年第12期757-762,共6页Tianjin Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81202877)

摘　　要：[目的]研究甘草素在大鼠体内的代谢产物。[方法]采用静脉注射(iv)、灌胃(ig)两种给药方式,剂量20 mg/kg,收集大鼠血浆、尿液、粪便、胆汁,应用UPLC-Q-TOF/MS法鉴定甘草素的代谢产物。[结果]血浆、尿液、粪便、胆汁样品中共鉴定15种代谢产物,主要有异甘草素、(异)甘草素葡萄糖醛酸结合物和硫酸结合物、二氢异甘草素、二氢异甘草素硫酸结合物、异甘草素甲基化及甘草素脱氢的代谢产物;其主要代谢途径为异构化、葡萄糖醛酸化、硫酸化、甲基化、脱氢反应。[结论]构建的UPLC-Q-TOF/MS法可用于大鼠体内甘草素代谢产物的研究。[Objective] To systemically study metabolites of liquirtigenin in rats, an UPLC-Q-TOF/MS method was developed. [Methods] UPLC-Q-TOF/MS method was employed to identify metabolites of liquirtigenin in plasma, urine, feces and bile samples from rats ＇after iv and ig administration of liquirtigenin （20 mg/kg）. [Results] A total of 15 metabolites were characterized in those biologieal samples, including isoliquirtigenin, glucuronides and sulfates of （iso）liquirtigenin, davidigenin and its sulfates, methylated isoliquirtigenin, dehydrogenated liqnirtigenin. The main metabolic pathways of liquirtigenin were isomerization, glueu,＇onidation and sulfation, methylation, dehydrogenation. [Conclusion] The UPLC-Q-TOF/MS method established in this study is successfully applied to identify metabolites of liquirtigenin in rats.

关 键 词：甘草素 代谢产物 UPLC-Q-TOF/MS 异构化 葡萄糖醛酸化 硫酸化 

分 类 号：R284[医药卫生—中药学]