Motilin基因单核苷酸多态性与奶牛真胃变位易感性的关联分析  

Association Between a Single Nucleotide Polymorphism in the Motilin Gene and Abomasal Displacement in Dairy Cattle

作　　者：陆璐[1] 黄河天 郭刚 李锡智 马翀 刘林 王雅春[1] 俞英[1] 孙东晓[1] 张胜利[1] 曹杰[3] 张毅[1] 

机构地区：[1]中国农业大学动物科技学院,北京100193 [2]北京首农畜牧发展有限公司,北京100192 [3]中国农业大学动物医学院,北京100193

出　　处：《中国奶牛》2015年第9期1-4,共4页China Dairy Cattle

基　　金：国家科技支撑计划(2011BAD28B02;2012BAD12B01);中央高校基本科研业务费专项资金.(15054204;2014YJ004);教育部"分子育种技术"创新团队基金(IRT1191);现代农业产业技术体系建设项目(CARS-37)

摘　　要：以北京地区11个牧场的494头中国荷斯坦牛为试验材料,采用病例-对照(case-control)设计,研究了motilin(MLN)基因第一外显子内转录因子结合位点g.90T>C多态性与奶牛真胃变位易感性的关联。结果共检测到TT、TC、CC三种基因型,基因型频率分布符合Hardy-Weinberg平衡(P>0.05)。将该位点3种基因型与真胃变位易感性进行关联分析,结果发现在一胎牛中关联显著(Cochran-Armitage趋势检验P<0.05;卡方检验P=0.09);与基因型TT相比,基因型TC和CC的比值比(OR值)分别为1.52和2.06,即易感性CC>TC>TT,显示C等位基因更易导致真胃变位。这些结果支持了MLN基因与荷斯坦牛真胃变位可能存在密切的关系。对g.90T>C位点T等位基因的选择,将有利于提高群体对真胃变位的遗传抗性。The association between a transcription factor binding site polymorphism （g.90T〉C） in the exon 1 of bovine MLN gene and abomasal displacement in dairy cattle was investigated using a case-control design. A total of 494 Chinese Holstein cows were collected from 11 herds in Beijing. Three kinds of genotypes （TT, TC, CC） were identified and no significant departure from Hardy-Weinberg equilibrium was detected （P〉0.05）. Significant association between the SNP and abomasal displacement was found for the first lactation cows （Cochran-Armitage trend test P〈0.05; chi-square test P=0.09）. Odds ratios （OR） for TC and CC were 1.52 and 2.06, respectively, with TT as reference, indicating the increasing susceptibility of cows with TT, TC, and CC. These results suggested a significant genetic association between MLN gene and abomasal displacement in Chinese Holstein cattle. Selection for the favorable T allele of g.90T〉C could potentially improve genetic resistance to abomasal displacement.

关 键 词：中国荷斯坦牛 MLN基因 真胃变位 Cochran-Armitage趋势检验 PCR-RFLP 

分 类 号：S823.3[农业科学—畜牧学]