几种物理化学技术在天然产物立体结构解析中的应用  

Application of several physicochemical techniques in natural products to elucidate stereochemistry

作　　者：李长伟[1] 崔承彬[1] 

机构地区：[1]抗毒药物与毒理学国家重点实验室,军事医学科学院毒物药物研究所,北京100850

出　　处：《国际药学研究杂志》2015年第6期811-828,共18页Journal of International Pharmaceutical Research

基　　金：国家自然科学基金资助项目(81573300;30973631);国家"重大新药创制"科技重大专项资助项目(2012ZX09301-003)

摘　　要：阐明天然产物的立体化学尤其是测定绝对构型,往往涉及天然产物结构研究的关键技术和方法,也是天然产物结构解析的技术难点所在。本文结合课题组近期研究工作和相关技术方法基本原理,归纳介绍我们利用钼试剂Mo2(OAc)4诱导CD(ICD,Snatzke法)、部分经典CD经验规则、改良Mosher法、计算ECD、改良Marfey分析法等几种技术解析阐明手性新天然产物立体化学的几个研究实例和我们应用相关技术方法的粗浅体会,以供相关研究者借鉴和参考。The difficulty in the structure elucidation of natural products is in the elucidation of stereochemistry,especially the determination of absolute configuration,which generally involves the key techniques of structure elucidation of natural products.This paper shows some examples of our work in elucidating the stereochemistry of new chiral natural products by the application of several physicochemical techniques,such as the dimolybdenum tetraacetate[Mo2（OAc）4]induced CD（ICD,Snatzke′s method）,empirical rules of classical CD,modified Mosher′s method,theoretical ECD calculation and modified Marfey′s analysis. The fundamental rules of the techniques and our experiences in the application of these techniques are also described for related researcher′s reference in relevant studies.

关 键 词：天然产物 立体化学 绝对构型 诱导CD 钼试剂四醋酸钼 Snatzke法 CD经验规则 计算ECD Mosher法 Marfey分析法 

分 类 号：O629[理学—有机化学]