First Insights into the Viral Communities of the Deep-sea Anoxic Brines of the Red Sea  

作　　者：Andr Antunes Intikhab Alam Marta Filipa Simoes Camille Daniels Ari J.S.Ferreira Rania Siam Hamza El-Dorry Vladimir B.Bajic 

机构地区：[1]Computational Bioscience Research Center (CBRC),Computer,Electrical and Mathematical Sciences and Engineering Division (CEMSE),King Abdullah University of Science and Technology (KAUST) [2]Red Sea Research Center (RSRC),Biological and Environmental Sciences and Engineering Division (BESE),King Abdullah University of Science and Technology (KAUST) [3]Biology Department,American University in Cairo

出　　处：《Genomics, Proteomics & Bioinformatics》2015年第5期304-309,共6页基因组蛋白质组与生物信息学报（英文版）

基　　金：the support through the KAUST baseline research funds to VBB;partially supported by the KAUST-AUC Global Collaborative Research Program

摘　　要：The deep-sea brines of the Red Sea include some of the most extreme and unique envi- ronments on Earth. They combine high salinities with increases in temperature, heavy metals, hydrostatic pressure, and anoxic conditions, creating unique settings for thriving populations of novel extremophiles. Despite a recent increase of studies focusing on these unusual biotopes, their viral communities remain unexplored. The current survey explores four metagenomic datasets obtained from different brine-seawater interface samples, focusing specifically on the diversity of their viral communities. Data analysis confirmed that the particle-attached viral communities present in the brine-seawater interfaces were diverse and generally dominated by Candovirales, yet appearing distinct from sample to sample. With a level of caution, we report the unexpected finding of Phycodnaviridae, which infects algae and plants, and trace amounts of insect-infecting Iridoviridae. Results from Kebrit Deep revealed stratification in the viral communities present in the interface： the upper-interface was enriched with viruses associated with typical marine bacteria, while the lower-interface was enriched with haloviruses and halophages. These results provide first insights into the unexplored viral communities present in deep-sea brines of the Red Sea, represent- ing one of the first steps for ongoing and future sampling efforts and studies.The deep-sea brines of the Red Sea include some of the most extreme and unique envi- ronments on Earth. They combine high salinities with increases in temperature, heavy metals, hydrostatic pressure, and anoxic conditions, creating unique settings for thriving populations of novel extremophiles. Despite a recent increase of studies focusing on these unusual biotopes, their viral communities remain unexplored. The current survey explores four metagenomic datasets obtained from different brine-seawater interface samples, focusing specifically on the diversity of their viral communities. Data analysis confirmed that the particle-attached viral communities present in the brine-seawater interfaces were diverse and generally dominated by Candovirales, yet appearing distinct from sample to sample. With a level of caution, we report the unexpected finding of Phycodnaviridae, which infects algae and plants, and trace amounts of insect-infecting Iridoviridae. Results from Kebrit Deep revealed stratification in the viral communities present in the interface： the upper-interface was enriched with viruses associated with typical marine bacteria, while the lower-interface was enriched with haloviruses and halophages. These results provide first insights into the unexplored viral communities present in deep-sea brines of the Red Sea, represent- ing one of the first steps for ongoing and future sampling efforts and studies.

关 键 词：Viral diversity METAGENOMICS Brine-seawater interface Caudovirales Red Sea 

分 类 号：Q938.8[生物学—微生物学]