白花蛇舌草的有效成分2-羟基-3-甲基蒽醌通过Fas/FasL信号通路诱导卵巢癌细胞凋亡  

2-Hydroxy-3-methylanthraquinone from Hedyotis diffusa Willd induces the apoptosis of HO-8910 cells through Fas/FasL signaling pathway

作　　者：万小旭 张行[2] 王佳贺[1] 

机构地区：[1]中国医科大学附属盛京医院老年病科,沈阳110004 [2]中国医科大学附属盛京医院喉科,沈阳110004

出　　处：《实用药物与临床》2015年第12期1405-1409,共5页Practical Pharmacy and Clinical Remedies

基　　金：国家自然基金资助项目(81101224)

摘　　要：目的研究中药白花蛇舌草的有效成分2-羟基-3-甲基蒽醌对人卵巢癌细胞HO-8910生长的抑制作用和凋亡的影响。方法不同浓度的2-羟基-3-甲基蒽醌与人卵巢癌细胞HO-8910分别培养0、24、48、72 h,用台盼蓝染色法测定HO-8910细胞存活率;以Annexin V FITC/PI双染流式细胞仪检测HO-8910细胞的凋亡率;Western blot法检测Fas和Fas L蛋白的表达;采用荧光比色法测定Caspase-3和Caspase-8蛋白酶的活性。结果随着2-羟基-3-甲基蒽醌浓度的增加及作用时间的延长,药物对HO-8910细胞的生长有明显的抑制作用;HO-8910细胞的凋亡率亦随之增高,且呈药物浓度及时间依赖关系;2-羟基-3-甲基蒽醌作用HO-8910细胞后Fas和Fas L的蛋白表达水平逐渐上升;Caspase-3和Caspase-8蛋白酶的表达亦逐渐增强;Caspase-3和Caspase-8抑制剂能够抑制2-羟基-3-甲基蒽醌诱导的HO-8910细胞凋亡。结论 2-羟基-3-甲基蒽醌可以诱导HO-8910细胞凋亡,Fas/Fas L通路在2-羟基-3-甲基蒽醌诱导的HO-8910细胞凋亡过程中起重要作用。Objective To explore the effects of 2-Hydroxy-3-methylanthraquinone fromHedyotis diffusa on proliferation and apoptosis of human ovarian cancer cells HO-8910 and the expressions of Fas / FasL proteins. Methods HO-8910 cells were cultured with different concentrations of 2-Hydroxy-3-methylanthraquinoe at different time points. Cell proliferation was detected by trypan blue stain; cell apoptosis was examined by flowcytometry; and the Fas / Fas L protein was detected by Western blot assay. The activity of caspases was detected by colorimetric assay. Results 2-Hydroxy-3-methylanthraquinoe induced apoptosis of HO-8910 cells in a time- and dose-dependent manner.The expression of Fas and Fas L proteins increased after treatment by 2-Hydroxy-3-methylanthraquinoe. Moreover,treatment of HO-8910 cells with 2-Hydroxy-3-methylanthraquinoe resulted in activation of caspase-3 and caspase-8. The caspases inhibitors decreased apoptosis in HO-8910 cells. Conclusion 2-Hydroxy-3-methylanthraquinoe can enhance the apoptosis of HO-8910 cells through Fas / Fas L signaling pathway.

关 键 词：白花蛇舌草 2-羟基-3-甲基蒽醌 HO-8910 凋亡 

分 类 号：R285[医药卫生—中药学]