HPLC法同时测定鸡眼草中染料木素和山柰酚的含量  

Simultaneous determination of Genistein and Kaempferol from Kummerowia striata by HPLC

作　　者：靳鑫[1] 秦晓红[2] 许莉 宿冰 穆晓婷[1] 刘豪华[1] 李亚丽[1] 祝峥[1] 张东方[1] 

机构地区：[1]中国医科大学药学院,沈阳110001 [2]辽宁省建平县医院药局,朝阳122400 [3]辽宁省中药研究所,沈阳110041

出　　处：《实用药物与临床》2015年第12期1476-1478,共3页Practical Pharmacy and Clinical Remedies

摘　　要：目的建立HPLC同时测定鸡眼草药材中染料木素和山柰酚含量的方法。方法采用RPHPLC法。色谱柱为Diamons C_（18）（150 mm×4.6 mm,5μm）,流动相为甲醇-体积分数0.1%磷酸水溶液（体积比为72∶28）,流速为1.0 m L/min,检测波长为370 nm,柱温为30℃。结果染料木素和山柰酚质量浓度分别在0.50~4.02 mg/L和0.15~1.20 mg/L内与峰面积呈良好的线性关系,平均回收率分别为99.7%和100.9%,RSD分别为0.1%和0.7%（n=6）。结论建立的方法简便,准确,重现性好,可用于鸡眼草药材中染料木素和山柰酚的含量测定。Objective To establish a method for the simultaneous quantitative analysis of genistein and kaempferol in the aerial parts of Kummerowia striata with HPLC. Methods The chromatographic column was Diamonsil C_（18）（ 150 mm× 4. 6 mm,5 μm）; the mobile phase was methnol and aqueous solution containing 0. 1 % phosphoric acid with the volume ratio of 72∶ 28 and the flowrate was 1. 0 mL / min; the UV detection wavelength was 370nm; the oven temperature was 30 ℃. Results The linear ranges of genistein and kaempferol were 0. 50 ~ 4. 02 mg / L and 0. 15 ~ 1. 20 mg / L,respectively. The average recoveries（ n = 6） of genistein and kaempferol were 99. 7% with RSD of 0. 1%,100. 9% with RSD of 0. 7%,respectively. Conclusion The method is simple and accurate,it can be used to determine genistein and kaempferol in the aerial parts of Kummerowia striata.

关 键 词：鸡眼草 染料木素 山柰酚 高效液相色谱 含量测定 

分 类 号：R284[医药卫生—中药学] O657.72[医药卫生—中医学]