反向蛋白质微点阵对怀孕女性TORCH感染的检测  

Detection of TORCH infection in pregnant women by using reverse phase protein array

作　　者：何文军[1] 唐芳 李涛[1] 吴子安[1] 吴新忠[1] 江帆 左连东 余婷玉[1] 谭志容[1] 徐宁[1] 

机构地区：[1]广东省中医院检验科,广东广州510120 [2]广州市人口和计划生育科学研究所,广东广州510410

出　　处：《国际检验医学杂志》2015年第24期3522-3524,共3页International Journal of Laboratory Medicine

基　　金：广东省人口和计划生育委员会科研项目(2012338)

摘　　要：目的评价反向蛋白质微点阵方法是否能用于怀孕女性TORCH感染的检测。方法建立检测TORCH感染的反向蛋白质微点阵方法,比较反向蛋白质微点阵和酶联免疫吸附试验(ELISA)方法检测2 000份怀孕女性的新鲜血清TORCH感染的阳性符合率,评价反向蛋白质微点阵检测TORCH的可行性。结果建立的反向蛋白质微点阵方法与ELISA方法对TORCH感染检测的阳性符合率分别为100.0%、91.1%、97.2%、91.3%和93.0%,通过蛋白芯片法与ELISA法各项指标检测结果均具有较好的一致性(P>0.05),但反向蛋白质微点阵对风疹病毒、巨细胞病毒和疱疹病毒的检测率高于相对应的ELISA方法。结论反向蛋白微点阵法检测TORCH感染,具有简便、快速,敏感性较高和特异性强等优点,是临床优生优育辅助诊断的有效方法,未来值得推广使用。Objective To evaluate whether the reverse phase protein array（RPPA）method can be used for detecting TORCH infection in pregnant women.Methods The RPPA method was established for detecting TORCH infection.The positive coincidence rates of TORCH infection detected by the RPPA method and ELISA method in 2000 fresh serum samples from pregnant women were compared for evaluating the feasibility of RPPA in TORCH detection.Results The positive coincidence rates of established RPPA and ELISA for detecting TORCH infection was 100.0%,91.1%,97.2%,91.3% and 93.0% respectively,indicating that the detection results of various indexes by RPPA and ELISA had better consistency（P〉0.05）,but the positive detection rates of RPPA for Rubellavirus,CMV and HSV-1,2were higher than those of correspondent ELISA method.Conclusion RPPA method for detecting TORCH infection has the advantages of simpleness,rapidness,high sensitivity and strong specificity,is an effective method of auxiliary diagnosis for bearing and rearing better children in clinical,and is worthy of being promoted and used in the future.

关 键 词：反向蛋白质微点阵 TORCH感染 酶联免疫吸附试验 

分 类 号：R446.6[医药卫生—诊断学]