铜绿假单胞菌耐药性检测及整合子分布  

The detection of drug resistance and prevalence of integrons in clinical isolates of Pseudomonas aeruginosa

作　　者：于魁魁 谢凯[1] 

机构地区：[1]徐州市中心医院检验科,江苏徐州221100

出　　处：《国际检验医学杂志》2015年第24期3547-3549,共3页International Journal of Laboratory Medicine

摘　　要：目的了解临床分离铜绿假单胞菌(PA)的耐药特征及耐药整合子分布,探讨整合子携带与耐药的关系。方法收集并保存该院临床分离的非重复PA菌株260株;药物敏感试验采用纸片法(K-B法);药物敏感结果统计分析采用WHONET5.0软件;整合子检测采用聚合酶链式反应(PCR)方法。结果临床分离的260株PA菌株对14种抗菌药物耐药率由低到高依次为:亚胺培南(18.5%)、阿米卡星(18.8%)、头孢吡肟(20.3%)、哌拉西林/他唑巴坦(21.5%)、头孢他啶(22.6%)、氨曲南(31.9%)、妥布霉素(31.9%)、头孢哌酮(38.1%)、庆大霉素(38.8%)、哌拉西林(41.9%)、环丙沙星(49.2%)、头孢噻肟(61.9%)和磺胺甲噁唑/甲氧苄啶(70.4%);检出Ⅰ类整合子阳性菌株109株(41.9%),Ⅱ类整合子4株(1.5%),未检测出Ⅲ类整合子;发现除多黏菌素外,Ⅰ类整合子检测阳性菌株对不同种类抗菌药物的耐药率明显高于Ⅰ类整合子检测阴性菌株,差异有统计学意义(P<0.05)。结论该地区临床分离PA菌株携带整合子类型为Ⅰ、Ⅱ型,菌株携带整合子与该地区PA耐药密切相关。Objective To investigate the drug resistance and prevalence of integrons in clinical isolates of Pseudomonas aeruginosa（PA）and to analyze the correlation between presence of integrons and drug resistance.Methods A total of 260non-repetitive clinical isolates of PA were obtained from this Hospital;antimicrobial susceptibility testing was performed by using the K-B disk diffusion methods;statistical analysis was performed by using the WHONET5.0software.Integrons were detected by using polymerase chain reaction（PCR）.Results Among the 260 clinical isolates of PA,the resistance rates against 14 antibacterial agents from low to high were imipenem（18.5%）,amikacin（18.8%）,cefepime（20.3%）,piperacillin/tazobactam（21.5%）,ceftazidime pentahydrate（22.6%）,aztreonam（31.9%）,tobramycin（31.9%）,cefoperazone（38.1%）,gentamicin（38.8%）,piperacillin（41.9%）,ciprofloxacin（49.2%）,cefotaxime（61.9%）and sulfamethoxazole/trimethoprim（70.4%）,respectively.A total of 109 isolates presented ClassⅠintegrons and 4isolates presented classⅡintegrons,while no classⅢintegrons were found in any isolates.Except for polymyxin,the resistance rates of class Ⅰ integrons positive isolates against antibacterial agents examined in this study were significantly higher than those of classⅠintegrons negative isolates（P〈0.05）.Conclusion The classⅠand classⅡintegrons are the prevalent types in the local region,which might be closely related to the drug resistance in clinical isolates of PA.

关 键 词：铜绿假单胞菌 整合子 耐药性 

分 类 号：R446.5[医药卫生—诊断学]