HPLC法同时测定香连化滞丸中10种成分  

Simultaneous determination of ten components in Xianglian Huazhi Pill by HPLC

作　　者：王晓玲[1] 赵力辉 

机构地区：[1]天津医科大学第二医院药学部,天津300211 [2]中国医学科学院生物医学工程研究所,天津300193

出　　处：《中草药》2015年第21期3190-3193,共4页Chinese Traditional and Herbal Drugs

摘　　要：目的建立同时测定香连化滞丸中芍药苷、甘草苷、厚朴酚、阿魏酸、橙皮苷、木香烃内酯、去氢木香烃内酯、辛弗林、黄芩苷、盐酸小檗碱10种成分的HPLC方法。方法采用RP-HPLC法,色谱柱为Waters Symmetry-C18（150 mm×4.6mm,3.5μm）;流动相为甲醇-乙腈-水（50∶45∶5,A）和甲醇-0.1%磷酸水溶液（4∶96,B）,梯度洗脱,体积流量1.0 m L/min;柱温45℃。结果待测定的10种指标成分中芍药苷在1.5~15.0μg/m L、甘草苷在0.5~5.0μg/m L、厚朴酚在1.0~10.0μg/m L、阿魏酸在0.5~5.0μg/m L、橙皮苷在12.5~125.0μg/m L、木香烃内酯在2.5~25.0μg/m L、去氢木香烃内酯2.5~25.0μg/m L、辛弗林在0.75~7.5μg/m L、黄芩苷在1.5~15.0μg/m L、盐酸小檗碱在0.75~7.5μg/m L线性关系良好;精密度良好,RSD均小于2.0%;重复性良好,RSD均小于2.0%;在室温条件下12 h内稳定;平均加样回收率在98.5%~101.4%,RSD均小于2.0%。结论方法准确、灵敏、可靠,重现性较好,可用于香连化滞丸的质量控制。Objective To develop an HPLC method for the simultaneous determination of paeoniflorin, liquiritin, magnolol, ferulic acid, hesperidin, costunolide, dehydrocostus lactone, synephrine, baicalin, and berberine in Xianglian Huazhi Pill. Methods The chromatographic separation was achieved on a Waters Symmetry-C18（150 mm × 4.6 mm, 3.5 μm） column with methanolacetonitrile-waters（50∶45∶5, A） and methanol-0.1% phosphoric acid（4∶96, B） as mobile phases for gradient elution, at the flow rate of 1.0 m L/min; The column temperature was 45 ℃. Results The results showed that the ten active components were well separated and showed good linearity, paeoniflorin（1.5 — 15.0 μg/m L）, liquiritin（0.5 — 5.0 μg/m L）, magnolol（1.0 — 10.0 μg/m L）,ferulic acid（0.5—5.0 μg/m L）, hesperidin（12.5—125.0 μg/m L）, costunolide（2.5—25.0 μg/m L）, dehydrocostus lactone（2.5—25.0μg/m L）, synephrine（0.75—7.5 μg/m L）, baicalin（1.5—15.0 μg/m L）, and berberine（0.75—7.5 μg/m L）. The precision was good and RSD was less than 2.0%. The repeatability was good, and RSD was less than 2.0%. The stability was good in 12 h. The average recoveries were between 98.5%—101.4%, and RSD was less than 2.0%. Conclusion The method is accurate, sensitive, credible,and repeatable. It can be applied to the quality control of Xianglian Huazhi Pill.

关 键 词：香连化滞丸 HPLC 芍药苷 甘草苷 厚朴酚 阿魏酸 橙皮苷 木香烃内酯 去氢木香烃内酯 辛弗林 黄芩苷 盐酸小檗碱 

分 类 号：R286.02[医药卫生—中药学]