壳聚糖微纳米粒作为鱼类疫苗佐剂的应用研究  

Chitosan Microparticles and Nanoparticles as Fish Vaccine Adjuvants

作　　者：董浩苏 吴海珍[1] 张元兴[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《华东理工大学学报（自然科学版）》2015年第6期792-797,845,共7页Journal of East China University of Science and Technology

基　　金：国家科技支撑计划(2012BAD17B00);国家高技术研究发展("863")计划(2014AA093604)

摘　　要：壳聚糖是由脱水-d-葡萄糖胺和脱水-N-乙酰-d-葡萄糖胺通过β-(1→4)-糖苷键连接而成的一种自然多糖,因其良好的生物相容性、生物可降解性和生物活性,作为生物材料被广泛应用于制药和医学领域。本研究以斑马鱼为实验动物,评价了壳聚糖微纳米粒作为鱼类疫苗佐剂的效果。结果表明:壳聚糖微粒作为佐剂具有较好的免疫增益作用,其微粒负载抗原蛋白ETAE_1227接种斑马鱼后的免疫效果与商业化油性佐剂Montanide ISA 763A效果相当,且注射接种壳聚糖微粒的斑马鱼伤口未见红肿与溃烂,即壳聚糖微粒作为鱼用佐剂具有更好的生物安全性。Chitosan is a linearβ-（1→4）-linked copolymer of anhydro-d-glucosamine and anhydro-Nacetyl-d-glucosamine.Owing to its biocompatibility,biodegradability and bioactivity,it is becoming a very attractive substance for diverse applications as a biomaterial in pharmaceutical and medical fields.In this study,the potential of chitosan microparticles and nanoparticles as fish vaccine adjuvants was evaluated using zebrafish model.Chitosan microparticles were found to be equipotent to commerical animal adjuvant Montanide ISA 763 Ain terms of immune effect and much safer.

关 键 词：鱼用疫苗佐剂 壳聚糖 微纳米粒 斑马鱼 

分 类 号：Q933[生物学—微生物学]