基于不同对照品的灵芝酸紫外及比色法分析  

Comparative Analysis of Ganoderic Acid in Ganoderma lucidum Mycelium and Fruit Body with the Ultraviolet Absorption Method and Colorimetric Method Using Different Standards

作　　者：刘盛荣[1] 戴金玉 张维瑞[1] 郑春源 余浩 高益槐 

机构地区：[1]宁德师范学院生物系,福建宁德352100 [2]安发(福建)生物科技有限公司,福建宁德352100

出　　处：《中国农学通报》2015年第35期104-109,共6页Chinese Agricultural Science Bulletin

基　　金：区域科技重大项目"灵芝三萜酸深层发酵提取技术研究及应用"(2014Y3006);校企合作项目"灵芝液体深层发酵研究"(AFSW-2014-003);校博士启动基金项目"智能菇房研制以及出菇示范"(2013Y008)

摘　　要：为优化灵芝酸紫外吸收及香草醛-高氯酸比色法分析,采用紫外可见光谱分析不同灵芝酸及其对照品光谱差异,并基于不同对照品的紫外吸收法和香草醛-高氯酸比色法分析灵芝发酵菌丝及子实体灵芝酸含量。结果显示:灵芝酸A、C、C2、E和赤芝酸A有相似的紫外可见光谱,形成一个波峰,254 nm附近有最大吸收波长;百里酚显示2个波峰,246 nm为波谷。熊果酸和齐墩果酸的香草醛-高氯酸显色反应紫外可见光谱相似,544 nm及552 nm分别有最大吸收;灵芝酸A显色反应弱且无吸收峰,不可作为灵芝酸对照品。以灵芝酸A和百里酚为对照品,紫外吸收法显示灵芝发酵菌丝灵芝酸含量分别为0.7 mg/100 mg和1.3 mg/100 mg,子实体分别为2.1 mg/100 mg和4.0 mg/100 mg;以熊果酸和齐墩果酸为对照品,香草醛-高氯酸比色法显示灵芝发酵菌丝灵芝酸含量分别为0.45 mg/100 mg和0.49 mg/100 mg,子实体分别为1.55 mg/100 mg和1.71 mg/100 mg。由此可知灵芝酸紫外吸收法和香草醛-高氯酸比色法分析以及紫外吸收法基于不同对照品的分析值有显著差异(P<0.05)。本研究对灵芝酸分析方法的优化和改进具有重要参考价值。To optimize ganoderic acid analysis of the ultraviolet absorption method and colorimetric method,ultraviolet-visible spectrum was used to examine differences among ganoderic acids（A, C, C2 and E）, lucidenicacid A and thymol using ultraviolet absorption method, and among ursolic acid, oleanolic acid, and ganodericacid A after the reaction with the color-developing agents vanillin and perchloric acid. After the extraction, theganoderic acid contents were analyzed and calculated using the two methods taking the mycelium and fruitingbodies of Ganoderma lucidum as test samples based on different standard curves. The results showed thatganoderic acids A, C, C2, E, and lucidenic acid A had similar spectrum with the formation of a peak with themaximum absorbance at around 254 nm. However, thymol formed two peaks, and the trough was clearlyobserved at 246 nm. As to the vanillin-perchloric acid colorimetric assay, ursolic acid had similar spectrumwith oleanolic acid with peaks at 544 nm and 552 nm, whereas almost no absorbance was recorded forganoderic acid A, indicating that it could not be used as a standard material in the colorimetric assay. With ganoderic acid A and thymol as standards, the ultraviolet absorption method indicated that the content ofganoderic acid was 0.7 mg/100 mg（dry biomass） and 1.3 mg/100 mg respectively in the mycelium, and2.1 mg/100 mg and 4.0 mg/100 mg in the fruit body. Based on ursolic acid and oleanolic acid standard curves,the colorimetric assay showed that ganoderic acid content was 0.45 mg/100 mg and 0.49 mg/100 mgrespectively in the mycelium, and 1.55 mg/100 mg and 1.71 mg/100 mg in the fruit body. By comparing theseresults, it could be concluded that there were significant differences in ganoderic acid content between theultraviolet absorbance and the colorimetric methods and among different standards. The present study isvaluable for optimization of ganoderic acid quantitative analysis.

关 键 词：灵芝 灵芝酸 紫外可见光谱 紫外吸收法 香草醛-高氯酸比色法 

分 类 号：S646.2[农业科学—蔬菜学]