柴苓护肝颗粒的优化工艺  

Optimizationprocess of Chailing Hugan granule

作　　者：杨瑾 莫国栋 宋凤兰[2] 李嘉良 曾维国 林丽 

机构地区：[1]广东省中山市第二人民医院药剂科,528400 [2]广东药学院医药化工学院

出　　处：《环球中医药》2015年第12期1455-1459,共5页Global Traditional Chinese Medicine

基　　金：中山市科技成果计划(医疗卫生)(2014A1FC150)

摘　　要：目的探讨柴苓护肝颗粒的工艺优化研究。方法通过正交实验以干膏得率和总皂苷含量为指标,对水提工艺和醇沉工艺分别进行了优化,水提部分对药材浸泡时间、煎煮时间、煎煮次数、加水量进行了考察,醇沉部分对醇沉浓度、醇沉时间、浓缩液相对密度进行了优选,筛选出最佳提取工艺。结果柴苓护肝颗粒的最佳水提工艺为加10倍量水,药材浸泡3小时,提取2次,每次提取2小时。最佳醇沉工艺为提取液浓缩至相对密度为1.16~1.20,加乙醇使含醇量为50%,静置36小时。结论该工艺稳定可行,合理可靠。Objective To discuss the optimization process of Chailing Hugan granule. Methods To optimize the water-extracting process and ethanol-extracting process respectively based on the indexes as dry extract yielding rate and total saponins contents by using orthogonal design. In water-extracting process,soaking time,decocting time,decocting frequencies and the additive quantity of water of herb decocting were observed. In ethanol-extracting process,alcohol concentration,soaking time and relative density of concentrated solution were optimized,in order to select the best extraction process. Results The optimization water-extracting process of Chailing Hugan granule was as follows： 10 times of water quantity was added,and herbs were soaked for 3 hours and extracted 2 times with 2 hours for each extraction. The optimization ethanol-extracting process of Chailing Hugan granule was as follows： adding ethanol to the ethanol content of 50%,placed statically for 36 hours and extracted the concentrated solution with relative density in the range of 1. 16 ~ 1. 20. Conclusion This process was stable,practical,reasonable and reliable.

关 键 词：柴苓护肝颗粒 正交设计 水提工艺 柴胡皂苷 

分 类 号：R284.2[医药卫生—中药学]