大气PM2.5对人支气管上皮细胞内钙稳态的影响  

Effect of PM2.5 on intracellular calcium homeostasis of 16HBE

作　　者：平飞飞 徐贞贞[1] 马晓燕[1] 崔井龙 张志红[1] 

机构地区：[1]山西医科大学公共卫生学院环境卫生学教研室,山西太原030001

出　　处：《环境与健康杂志》2015年第9期779-782,共4页Journal of Environment and Health

基　　金：山西省回国留学人员科研资助项目(2013-057);教育部留学回国人员科研启动基金(教外司留[2013]1792号)

摘　　要：目的研究大气PM2.5对人支气管上皮细胞(16HBE)内钙稳态的影响。方法以50、100μg/ml大气PM2.5对16HBE细胞进行染毒,以生理盐水为对照组,并用终浓度为100μg/ml的钙离子拮抗剂肝素钠进行干预,即设生理盐水对照组、50μg/ml PM2.5组、100μg/ml PM2.5组、肝素钠组、50μg/ml PM2.5+肝素钠组、100μg/ml PM2.5+肝素钠组,分别染毒3、6、24 h后,以流式细胞仪检测细胞内游离钙离子荧光强度。结果 100μg/ml PM2.5染毒16HBE 3 h后,细胞内钙离子荧光强度高于对照组和50μg/ml PM2.5组;当加入胞内游离钙离子拮抗剂肝素钠后,100μg/ml PM2.5+肝素钠组钙离子荧光强度低于100μg/ml PM2.5组。100μg/ml PM2.5染毒16HBE 6 h后,细胞内钙离子荧光强度高于对照组。PM2.5染毒16HBE 24 h后,50μg/ml PM2.5组、100μg/ml PM2.5组、50μg/ml PM2.5+肝素钠组、100μg/ml PM2.5+肝素钠组的细胞内钙离子荧光强度均高于对照组,且100μg/ml PM2.5组细胞内钙离子荧光强度高于50μg/ml PM2.5组。50、100μg/ml PM2.5染毒组随着染毒时间的延长,钙离子荧光强度升高,染毒6、24 h后的钙离子荧光强度均高于染毒3 h后,上述差异均有统计学意义(P<0.05)。结论大气PM2.5可能刺激16HBE细胞释放胞内钙库,导致胞内游离钙离子荧光强度增加。Objective To explore the influence of PM2.5 on intracellular calcium homeostasis of the 16 HBE cells. Methods The cells were exposed to PM2.5 at the doses of 50 wg/ml and 100 μg/ml for 3 h, 6 h and 24 h, respectively, normal saline （NS） as the control group and intervention group treated with calcium antagonist heparin of 100 μg/ml. The exposure groups were 50 μg/ml PM2.5, 100 μg/ml PM2.5, heparin group, 50 μg/ml PM2.5＋heparin and 100 μg/ml PM2.5＋heparin, respectively. After infecting the cells, the intensity of intracellular Ca2＋ fluorescence （[Ca2＋]i） was measured by flow cytometry. Results The [Ca2＋]i in 100 txg/ml PM2.5 exposure group were higher than those in control group and 50 μg/ml PM2.5 exposure group. The [Ca2＋]a in 100 μg/ml PM2.5＋heparin group were lower than those in 100 μg/ml PM2.5 group by intervening the cells with calcium antagonisfs heparin after 3 h exposure. The [Ca2＋] in 100 μg/ml PM2.5 exposure group were more enhanced compared with that in control group after 6 h exposure. The [Ca2＋]i in 50 μg/ml PM2.5 exposure group, 100μg/ml PM2.5 exposure group, 50 μg/ml PM2.5＋heparin group and 100 μg/ml PM2.5＋heparin group were higher compared with those in control group after 24 h exposure. Particularly, the [Ca2＋] in 100 μg/ml PM2.5 exposure group were higher than that in 50 μg/ml PM2.5 group. The [Ca2＋] were risen by the doses of 50 and 100 μg/ml of PM2.5 with the extension of exposure time. Compared with 3 h PM2.5 exposure group, the [Ca2＋]i were higher in 6 h and 24 h PM2.5 exposure groups. Such differences were statistically significant （P〈0.05）. Conclusion PM2.5 can cause the release of intracellular calcium pool by stimulating 16HBE cells, leading to the increase of intracellular free calcium concentration.

关 键 词：PM2.5 16HBE 细胞 钙离子 Fluo4-AM 

分 类 号：R994.6[医药卫生—毒理学]