海藻玉壶汤加减甘草-海藻反药药对不同组方中11种活性成分在正常大鼠体内的药动学比较研究  

Comparisons of pharmacokinetic profile of eleven bioactive components in Haizao Yuhu decoction modified with Haizao and Gancao anti-drug pair in normal rats

作　　者：张洋[1] 钱大玮[1] 潘瑛[1] 翟燕娟 周学平[2] 钟赣生[3] 朱振华[1] 段金廒[1] 

机构地区：[1]南京中医药大学江苏省方剂高技术研究重点实验室江苏省中药资源产业化过程协同创新中心,江苏南京210023 [2]南京中医药大学第一临床医学院,江苏南京210023 [3]北京中医药大学基础医学院,北京100092

出　　处：《中国中药杂志》2015年第23期4672-4679,共8页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(973)项目(2011CB505300;2011CB505306)

摘　　要：海藻玉壶汤是含有甘草-海藻反药药对的古代名方之一,用于治疗各种甲状腺疾病,其临床应用较为广泛。该文采用UPLC-TQ-MS对大鼠灌胃给药不同组方海藻玉壶汤中血中11种生物活性成分进行测定,同时比较不同组方之间11种活性成分药动学差异,探讨了海藻玉壶汤与甘草-海藻反药药对的相互影响。首次建立了甘草苷、柚皮苷、橙皮苷、贝母素甲、贝母素乙、甘草酸、甘草素、佛手柑内酯、川陈皮素、蛇床子素与甘草次酸同时测定的UPLC-MS/MS含量测定方法。结果发现,海藻可提高甘草酸的峰浓度,海藻玉壶汤中其他组分可能促进甘草中黄酮类成分在正常大鼠体内的吸收,却抑制了皂苷类成分的吸收,加快甘草中某些皂苷类成分的代谢;甘草与海藻反药药对可提高橙皮苷、佛手柑内酯、川陈皮素、蛇床子素与贝母素甲的生物利用度,减慢柚皮苷与贝母素甲的消除。Haizao Yuhu decoction（ HYD） is a formula that has been used for approximately 500 years and famous for its efficiency in treating thyroid-related diseases in traditional Chinese medicine（ TCM）. HYD was first presented by Chen Shi-gong in a famous surgical monograph named Waike Zhengzong during the Ming Dynasty. We conducted the research to investigate the possible pharmacokinetic profile of different prescriptions of HYD in rats,in order to reveal the interactions of Haizao and Gancao drug pair with other herbs in HYD. Liquiritin,naringin,hesperidin,peimine,peiminine liquiritigenin,glycyrrhizic acid,bergapten,nobiletin,osthole,glycyrrhetinic acid in blood samples were determined by UPLC-MS / MS. The result revealed that Haizao could enhance the peak concentration of glycyrrhizic acid. The other herbs in HYD may promote the absorption of flavonoids in Gancao in normal rats,but inhibit the absorption of saponins and accelerate their metabolism. Gancao and Haizao drug pair could enhance the bioavailability of hesperidin,peimine,bergapten,nobiletin and osthole and prolong the elimination of peimine and naringin.

关 键 词：海藻玉壶汤 甘草-海藻反药药对 UPLC-MS/MS 多成分 药动学影响 

分 类 号：R285.5[医药卫生—中药学]