纳米纤维家蚕丝蛋白软支架制备及性能  

Preparation and properties of nano-fiber, soft Bombyx mori silk protein scaffolds

作　　者：宁洪艳[1] 韩宏岩[1] 吕强[2] 许维岸[1] 

机构地区：[1]苏州大学基础医学与生物科学学院,苏州215123 [2]现代丝绸国家工程实验室/苏州大学,苏州215123

出　　处：《应用昆虫学报》2015年第6期1507-1512,共6页Chinese Journal of Applied Entomology

基　　金：国家自然科学基金(No.81071306)

摘　　要：【目的】通过调控蚕丝蛋白溶液的状态,可实现对蚕丝蛋白支架的力学及结构性能的调控,获得适宜于内皮细胞生长的支架材料,解决复杂组织修复中由于不利于组织长入和血管生长而导致组织功能无法修复的问题。【方法】向新鲜蚕丝蛋白溶液中添加蚕丝蛋白纳米纤维增加冻干支架的成孔性,基于丝蛋白自组装原理对丝素蛋白溶液进行浓缩处理增加分子间亲水作用力,获得不同状态的丝蛋白溶液,最后冷冻干燥,制备支架,观察蚕丝蛋白状态变化对支架性能的影响。【结果】实验结果表明,蚕丝蛋白溶液经浓缩处理可以降低支架Silk II的形成,获得较柔软支架。【结论】添加蚕丝蛋白纳米纤维并浓缩处理可以获得模量适宜内皮细胞生长的支架材料,为不同软组织的修复提供了良好的基质材料。[Objectives] Regulating the nature of silk protein solution allows the control of the mechanical and structural properties of silk protein scaffolds suitable for the growth of endothelial cells, and solves the problem of engineering thick complex organs and tissues that require functional vasculature. [Methods] To study the effect of different silk protein scaffold properties nanofiber-assisted lyophilization was used to restrain separate lamella formation in silk scaffolds. In addition, the structure of silk was controlled by adjusting silk-water interactions, thereby obtaining different silk protein solutions, and through preparing scaffolds by lyophilization. [Results] Increasing silk-water interactions resulted in reduced silk Ⅱ （β-sheet crystal） formation, which resulted in a decrease in scaffold formation. [Conclusion] Scaffolds with a suitable stiffness and porous structure for the growth of endothelial cells were obtained by increasing the solution concentration. These also provided a promising matrix for different soft tissue engineering and regenerative medicine.

关 键 词：蚕丝素蛋白 软组织 力学性能 冷冻干燥 二级结构 

分 类 号：R318.08[医药卫生—生物医学工程]