Establishment of Real-time Fluorescent Quantitative PCR for Pineapple mealybug wilt associated virus-3  

作　　者：Hu Jiayi Li Xianghong Luo Zhiwen Zhang Zhili Liu Zhixin He Fan Fan Hongyan 

机构地区：[1]Institute of Tropical Fruit Trees,Hainan Academy of Agricultural Science / Haikou Investigation Station of Tropical Fruit Trees,Ministry of Agriculture [2]College of Environment and Plant Protection,Hainan University [3]Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Science

出　　处：《Plant Diseases and Pests》2015年第4期6-10,18,共6页植物病虫害研究（英文版）

基　　金：Supported by Applied Research and Industrialization Projects of Key Science and Technology Plan of Hainan Province(ZDXM20130031);Special Fund for Agro-scientific Research in the Public Interest(201203021);Scientific Operation Fund of Hainan Province(QCY[2013]131);Natural Science Foundation of Hainan Province(QK[2013]32)

摘　　要：[ Objective ] The paper aimed to establish a real-time fluorescent quantitative PCR （qPCR） detection method for Pineapple mealybug wilt associated vi- rus-3 （ PMWaV3 ）. [ Method] Specific TaqMan probe and primers were designed and synthesized according to the conserved sequence of coat protein（CP） gene of PMWaV-3, and the standard curve was established after optimizing the amplification condition of qPCR. [ Result] The results showed that the method was specific for the detection of PMWaV-3, and the sensitivity of the present method was about 10 times higher compared to general RT-PCR ; the variation coefficients of intra- assay and inter-assay were less than 1.73, respectively. [ Conclusion] The qPCR is an easy, fast and reliable method for quantitative detection of PMWaV-3.[ Objective ] The paper aimed to establish a real-time fluorescent quantitative PCR （qPCR） detection method for Pineapple mealybug wilt associated vi- rus-3 （ PMWaV3 ）. [ Method] Specific TaqMan probe and primers were designed and synthesized according to the conserved sequence of coat protein（CP） gene of PMWaV-3, and the standard curve was established after optimizing the amplification condition of qPCR. [ Result] The results showed that the method was specific for the detection of PMWaV-3, and the sensitivity of the present method was about 10 times higher compared to general RT-PCR ; the variation coefficients of intra- assay and inter-assay were less than 1.73, respectively. [ Conclusion] The qPCR is an easy, fast and reliable method for quantitative detection of PMWaV-3.

关 键 词：Pineapple mealybug wilt associated virus-3 qPCR TaqMan probe Detection 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]