转基因奶牛与普通奶牛超数排卵效果的比较分析  

Comparative Analysis of Superovulation Responses in Transgenic Dairy Heifers and Ordinary Dairy Heifers

作　　者：朱玉林[1] 吕小青[1] 李艳华[1] 孙凤俊[1] 杨超[1] 戴蕴平[2] 王彦平[1] 赵凤茹[1] 丁方荣[2] 薛建华[1] 

机构地区：[1]北京奶牛中心,北京100192 [2]中国农业大学生物学院,北京100193

出　　处：《中国奶牛》2014年第23期16-18,共3页China Dairy Cattle

基　　金：国家重大专项课题"高品质转基因奶牛新品种培育"(2013ZX08007)和"提高牛胚胎生产效率的研究"

摘　　要：本文对20头转基因母牛与20头普通母牛超数排卵效果进行了比较分析。在20头超排转基因奶牛中,16头有超排反应,4头无超排反应,超排成功率为80%(16/20),平均获胚/卵总数为6.15±4.43枚,可用胚数为3.94±2.62枚,未受精卵数为1.50±2.19枚,退化胚胎数为0.69±0.78枚;20头对照组普通奶牛中,17头有超排反应,3头无超排反应,超排成功率为85%(17/20),平均获胚/卵总数为8.65±5.70枚,可用胚数为4.18±3.11枚,未受精卵数为1.47±1.12枚,退化胚胎数为3.00±4.24枚。统计分析表明,转基因奶牛的获胚/卵总数、可用胚数、未受精卵数与普通奶牛均无显著差异(P>0.05),转基因奶牛的退化胚胎数显著低于普通奶牛(P<0.05)。本试验结果说明转入改变乳成分的外源基因不影响奶牛的超数排卵效果。In this study, we compared the superovulation responses between transgenic dairy heifers and ordinary dairy heifers. Of the transgenic dairy heifers, 16 out of 20 responded to superovulation treatment. On average, the total number of embryos/ova and transferable embryos, unfertilized ova, degenerated embryos produced in transgenic dairy heifers were 6.15±4.43（mean±SEM） and 3.94±2.62, 1.50±2.19, 0.69±0.78, respectively. Of the ordinary dairy heifers, 17 out of 20 responded to superovulation treatment. On average, the total number of embryos /ova and transferable embryos, unfertilized ova, degenerated embryos produced in transgenic dairy heifers were 8.65±5.70 and 4.18±3.11, 1.47±1.12, 3.00±4.24, respectively. There was no significant difference between transgenic dairy heifers and ordinary dairy heifers in the total number of embryos/ova and transferable embryos, unfertilized ova（P〉0.05）.Tbere was a decrease in the number of degenerated embryos in transgenic dairy heifers（P〈0.05）. The results showed that transfer foreign gene to dairy heifers had no effect on the responses to superovulation treatment.

关 键 词：转基因奶牛 超数排卵 胚胎 

分 类 号：S823.3[农业科学—畜牧学]