参松养心胶囊对心梗后心肌的保护作用及机制研究  

Myocardial protective effects of shensongyangxin capsule after myocardial infarction and the possible mechanisms

作　　者：刘自强[1] 王晞[1] 党松[1] 

机构地区：[1]武汉大学人民医院心内科,湖北武汉430060

出　　处：《海南医学》2015年第24期3589-3592,共4页Hainan Medical Journal

基　　金：国家重点基础研究发展计划(973计划)(编号:2012CB518604)

摘　　要：目的探讨参松养心胶囊(SSYX)对心肌梗死后心律失常发生和心功能不全的影响及其作用机制。方法将40只新西兰大耳白兔行冠状动脉前降支结扎术后随机分为正常对照组(Control组)10只、心梗组(MI组)10只、心梗+胺碘酮组(MI+A组,0.4 g·kg^(^(-1))·d^(^(-1)))10只、心梗+参松组(MI+S组,0.5 g·kg^(^(-1))·d^(^(-1)))10只。正常对照组开胸后在冠状动脉下穿线,但不结扎。术后,分别以普食、普食+胺碘酮粉末、普食+参松养心胶囊原粉喂食。8周后将家兔处死,在心梗周边区取材,检测各组白兔缝隙连接蛋白43(CX43)、钠钙交换体(NCX)和兰尼碱受体蛋白(Ry R2)m RNA的相对表达量。结果与Control组比较,MI组CX43、Ry R2 m RNA相对表达量显著下降(P<0.05),而NCX m RNA相对表达量虽然有所下降,但其差异无统计学意义(P>0.05);与MI组比较,MI+A组CX43、Ry R2 m RNA相对表达量显著升高(P<0.05),MI+S组CX43、NCX、Ry R2 m RNA的相对表达量也显著升高(P<0.05);与MI+A组比较,MI+S组CX43、NCX m RNA的相对表达量均显著升高(P<0.05)。结论参松养心胶囊可以改善心肌梗死后心律失常以及心功能不全,其机制可能与调控各钙离子通道的开放,抑制钙超载有关。Objective To explore the effect of shensongyangxin capsule（SSYX） on arrhythmia and cardiac insufficiency after myocardial infarction（MI） in rabbits. Methods Forty New Zealand White rabbits were randomly assigned into control group, MI group, MI＋amiodarone group（MI＋A group, with the dose of amiodarone at0.4 g·kg^（-1）·d^（-1）） and MI＋SSYX group（MI＋S group, with the dose of SSYX at 0.5 g·kg^（-1）·d^（-1）） after successful establishment of model of MI, each with 10 rabbits. The control group applied stitches at coronary artery without ligation. The other three groups applied routine food, routine food＋amiodarone, routine food＋SSYX after surgery, respectively. After eight weeks, ryanodine receptor 2（Ry R2）, Connexin43（CX43）, sodium-calcium exchanger（NCX） m RNA expression in peri-infarcted area were examined. Results Compared with control group, CX43, Ry R2 m RNA relative expression significantly decreased in MI group（P〈0.05）, but NCX m RNA relative expression showed slight decrease without statistically significant difference（P〉0.05）. Compared with MI group, CX43, Ry R2 m RNA relative expression significantly increased in MI＋A group（both P〈0.05）, and CX43, NCX, Ry R2 m RNA relative expression significantly increased in MI＋S group（all P〈0.05）. Compared with MI ＋ A group, CX43, NCX, m RNA relative expression significantly increased in MI＋S group（all P〈0.05）. Conclusion SSYX can improve arrhythmia and cardiac dysfunction after myocardial infarction, which may be related to the open of calcium ion channels and the inhibition of calcium overload.

关 键 词：参松养心胶囊 心肌梗死 钙通道 缝隙连接蛋白43 钠钙交换体 兰尼碱受体蛋白 

分 类 号：R542.22[医药卫生—心血管疾病]