中药“凤香洗液”对伴HPV感染的CIN患者宫颈局部IL-2和IL-4的影响  

Effects of traditional Chinese medicine“ Fengxiang Lotion”on local cervical IL-2 and IL-4 in patients of cervical intraepithelial neoplasia with HPV infection

作　　者：李小宁[1] 贺丰杰[1] 吉喆 

机构地区：[1]陕西中医学院附属医院妇科,陕西咸阳712021 [2]西安西电集团医院妇科,陕西西安710077

出　　处：《海南医学》2015年第24期3604-3607,共4页Hainan Medical Journal

基　　金：陕西省教育厅自然科学专项(编号:2013JK0799)

摘　　要：目的观察中药"凤香洗液"对伴有HPV感染的CINⅠ、CINⅡ患者阴道局部免疫状态的影响及治疗效果。方法选取2013年7月至2015年2月于陕西中医学院附属医院、西安西电集团医院门诊就诊,经阴道镜下宫颈活检病理证实为CINⅠ、CINⅡ且伴有高危型HPV感染的患者共60例,其中CINⅠ30例,CINⅡ30例,对照组为同期健康体检的妇女30例。CINⅠ组接受3个疗程的"凤香洗液"治疗。应用ELISA方法测定各组人群用药前后阴道灌洗液辅助性T淋巴细胞(Th细胞)1、2细胞因子水平,其中Thl细胞因子以白细胞介素2(IL-2)表示,Th2细胞因子以IL-4表示。结果 CINⅠ组、CINⅡ组患者阴道灌洗液中IL-2表达水平显著低于对照组(P<0.01),CINⅠ组、CINⅡ组IL-4表达水平明显高于对照组(P<0.05),CINⅠ组、CINⅡ组患者IL-2/IL-4比值明显低于对照组(P<0.05),且这些变化在CINⅡ组较CINⅠ组更明显(P<0.05);CINⅠ组在用药后IL-2水平较治疗前明显升高(P<0.05),IL-4较治疗前明显降低(P<0.05),IL-2/IL-4比值升高(P<0.01)。结论 CIN患者阴道灌洗液中IL-2表达下降,IL-4表达升高,IL-2/IL-4降低,且这些改变与CIN的严重程度相一致;凤香洗液可能通过调节Th1/Th2的免疫平衡来阻断疾病的进展。Objective To observe the effects of traditional Chinese medical compound＂Fengxiang Lotion＂on the local vaginal immune state for patients of cervical intraepithelial neoplasia（CIN） with human papillomavirus（HPV） infection. Methods Sixty patients with high risk HPV infection diagnosed with cervical biopsy using colposcopy in the Affiliated Hospital of Shaanxi University of Chinese Medicine and Xi＇an XD Group Hospital from July2013 to February 2015 were selected in the study, which were divided into CINⅠ and CIN Ⅱ group（n=30 each）.Thirty healthy women coming for medical examination during the same period served as the control group. CINⅠgroup received Fengxiang Lotion for treatment, while the other two groups not. The levels of interleukin（IL）-2and IL-4 in cervical-vaginal lavage solution, which were synthesized by type 1 and type 2 helper T cells respectively, were tested by ELISA. Results（1） IL-2 levels of CINⅠgroup and CINⅡ group were significantly lower than that of the control group（P〈0.01）, while IL-4 levels of CIN Ⅰ group and CIN Ⅱ group were significantly higher（P〈0.05）. The ratios of IL-2 to IL-4 in CINⅠ group and CINⅡ group were significantly lower than that of the control group（P〈0.05）. What＇s more, all these changes was more significant in CIN Ⅱ group than CINⅠ group（P〈0.05）.（2） In CINⅠ group, the IL-2 level increased significantly after medication（P〈0.05）, while IL-4 decreased significantly（P〈0.05）, with the ratio of IL-2 to IL-4 increased significantly（P〈0.01）. Conclusion The IL-2 levels and IL-2/IL-4 ratio in CIN patients with HPV decreased, while IL-4 increased. These changes were consistent with the severity of CIN.＂Fengxiang Lotion＂may block the progression of CIN through regulating the balance of Th1 and Th2.

关 键 词：凤香洗液 宫颈上皮内瘤变 人乳头瘤病毒 白介素-2 白介素-4 

分 类 号：R711.74[医药卫生—妇产科学]