人源化NOD小鼠中CD8^+CD25^+Tregs的频率和功能研究  

The frequencies and function of CD8^+CD25^+ Tregs in humanized NOD mice

作　　者：买文丽[1] 韩清娟[2] 曹文轩[2] 陈晓玲[3] 冯志强[2] 王莉[3] 张梦军[2] 

机构地区：[1]川北医学院生理教研室,南充637000 [2]第三军医大学药物分析与分析化学教研室,重庆400038 [3]第三军医大学全军免疫学研究所,重庆400038

出　　处：《免疫学杂志》2016年第1期7-12,共6页Immunological Journal

基　　金：国家自然科学基金(31100653;31570931)

摘　　要：目的观察分析人源化NOD小鼠体内CD8+CD25+T细胞的频率及功能,探讨CD8+CD25+T细胞在人源化NOD小鼠的自身免疫进程中的作用。方法采用流式细胞术检测人源化NOD小鼠脾、胰腺引流淋巴结、腹股沟淋巴结中CD8+CD25+T、CD8+CD25+Foxp3+T细胞的频率及脾CD8+CD25+T细胞的表型和细胞因子分泌谱,并采用3H-Td R掺入法检测脾CD8+CD25+T细胞的免疫抑制功能。结果人源化NOD小鼠胰腺引流淋巴结中CD8+CD25+T和CD8+CD25+Foxp3+T细胞频率显著高于脾和腹股沟淋巴结中这2亚群的频率;与CD8+CD25-T细胞相比,脾CD8+CD25+T细胞高表达Foxp3、CTLA-4、CD103、CD62L,同时低分泌细胞因子IFN-γ及高分泌IL-17A;人源化NOD小鼠脾CD8+CD25+T细胞能有效地抑制效应性CD4+CD25-T细胞的增殖。结论人源化NOD小鼠的CD8+CD25+T细胞是一群具有免疫抑制功能的Tregs,并且高表达Tregs的表型和细胞因子谱,提示CD8+CD25+Tregs可能在外周和胰腺局部参与了人源化NOD小鼠T1D的免疫耐受进程。To evaluate the role of CD8^＋CD25^＋T cells in the process of autoimmunity of humanized NOD mice,the function and frequency changes of CD8^＋CD25^＋T cells in the process were observed. Flow cytometry wasemployed to analyze the CD8^＋CD25^＋T and CD8^＋CD25^＋Foxp3＋T cell frequencies of pancreas drainage lymph gland（PDLN）, inguinal lymph node（ILN）, and spleen; differences of phenotype and cytokine profile between splenic CD8^＋CD25^＋and CD8^＋CD25-T cells were also analyzed by flow cytometry. Data showed CD8^＋CD25^＋and CD8^＋CD25^＋Foxp3＋T cell frequencies of PDLN were higher than those of ILN and spleen. Compared with CD8^＋CD25-T cells, theCD8^＋CD25^＋T cell subpopulation harbored a typical Treg phenotype with high expression of Foxp3, CD103, CD62 Land CTLA-4, and inversely low level of IFN-γ and high level of IL-17 A. Furthermore, splenic CD8^＋CD25^＋T cellssignificantly inhibited the proliferation of CD4＋CD25-T cells. These results demonstrated that splenic CD8^＋CD25^＋Tcells in humanized NOD mice expresse classical Treg phenotype and cytokine profile and has significantimmunosuppressive function, thus might play an important immunosuppressive role in autoimmunity process ofhumanized NOD mice.

关 键 词：CD8+CD25+Tregs 人源化NOD小鼠 频率 功能 

分 类 号：R392.6[医药卫生—免疫学]