维生素E在体内外具有抗氧化作用  

Vitamin E has antioxidant effect both in vivo and in vitro

作　　者：仓宝成[1] 宫璀璀[1] 王莉[1] 何佩[1] 赖泽仁[1] 

机构地区：[1]中国人民解放军第一五三中心医院,河南郑州450042

出　　处：《基础医学与临床》2016年第1期80-84,共5页Basic and Clinical Medicine

摘　　要：目的探讨维生素E在体内外的应激损伤。方法体外实验:人肝RBL细胞系根据H_2O_2损伤及损伤前后给予维生素E分为4组,对照组(C)、H_2O_2损伤组(Ec)、H_2O_2损伤前(Eb)及后(Ea)给维生素E组;体内实验:将20只清洁级雄性Wistar大鼠分为对照组和大及小剂量维生素E组,每天进行一次35和15 mg/kg溶液2 m L灌胃,连续3 d。体外实验应用MTT和TUNEL法检测细胞存活率和凋亡率,免疫印迹和免疫组化方法检测细胞中NF-κB、Hsp-70、Bcl-2、Bax及caspase-3的表达水平;体内实验应用生化法检测3和6 d后血浆内T-AOC、SOD、GSH和MDA的水平。结果 H_2O_2损伤组(Ec)细胞凋亡率增加(P<0.01),细胞内Bax、Hsp-70、NF-κB及caspase-3显著升高(P<0.01),而Bcl-2显著下降(P<0.01),维生素E干预后能显著缓解上述变化(P<0.01),H_2O_2损伤前干预效果优于后干预。灌胃后3 d血浆中T-AOC、SOD、GSH的水平增高,MDA降低(P<0.01),6 d后其相关指标变化更加显著(P<0.05)。结论维生素E可通过调节人肝RBL细胞相关蛋白表达水平和Wistar大鼠血浆中抗氧化酶体系而发挥抗氧化作用。Objective To explore the antioxidant effect of vitamin E on both in the rat in vivo and in hepatic RBL cells in vitro. Methods The cultured human hepatic RBL cells were exposed to H2O2 as an oxidant and treated with0. 4m M doze of Vitamin E before or after exposure of H2O2. The cell survival and apoptosis were detected by MTT and TU-NEL assays. The expression level of NF-k B,Bcl-2,Bax,Hsp-70 and caspase-3 was determined by immunohistochmistry and Western blot. 20 clean level male Wistar rats were divided into control group and the big and small doses of vitamins E35 and 15 mg / kg 2 mL lavage solution,1 time a day,a total of three times,the biochemical method was performed to detect within the plasma after 3 and 6 d T-AOC,SOD,GSH and MDA levels.Results H2O2 damage group（ Ec） apoptosis rate increased（ P〈0. 01）,intracellular Bax,HSP-70,the NF-kappa B and caspase 3 significantly higher（ P〈0. 01）,while the Bcl-2 decreased significantly（ P〈0. 01）,vitamin E after the intervention can significantly alleviate the above changes（ P〈0. 01）,H2O2 damage before intervention was milder than that after intervention. Lavage after 3 d plasma T-the AOC,SOD,higher level of GSH,MDA lowered（ P〈0. 01）,and 6 d after the relevant index change was more significant（ P〈0. 05）. Conclusions The resultsshowed that the vitamin E displays anti-oxidant capacity through upregulating the antioxidant enzyme system and regulating the expression of relevant proteins.

关 键 词：维生素E H2O2 抗氧化酶体系 抗凋亡信号传导 

分 类 号：R96[医药卫生—药理学]