CAST基因5'调控区的多态性与鸡肉质性状的关联性分析  

Associations of Calpastatin Gene polymorphism in the 5' Regulatory Region with Meat Quality Traits in Chicken(Gallus gallus)

作　　者：李春苗[1] 黎寿丰[1] 赵振华[1] 黄华云[1] 王钱保[1] 薛龙岗[1] 

机构地区：[1]中国农业科学院家禽研究所,扬州225125

出　　处：《农业生物技术学报》2016年第1期76-82,共7页Journal of Agricultural Biotechnology

基　　金：现代农业产业技术体系建设专项(No.CARS-42-Z06);江苏省科技支撑项目(No.BE2013386);江苏省科技支撑项目(No.BE2014364);江苏省农业三新工程项目(No.SXGC(2014)285);江苏省重点实验室项目(No.K12039)

摘　　要：为探讨钙蛋白酶抑制蛋白(Calpastatin,CAST)基因5'调控区的多态性与鸡肉质性状的关系,本研究以中国农业科学院家禽研究所选育的F、D、Y、B和S3等5个优质肉鸡(Gallus gallus)品系为试验素材,利用聚合酶链式反应限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)技术检测CAST基因的SNPs,并分析其多态性与肌肉p H值、维生素B1、肉色、系水力、肌内脂肪和肌纤维密度之间的关系。结果显示,在距CDS-198位点发现1个突变位点(G→A),G-198A位点检测到3种基因型GG、GA和AA,相关分析显示,AA基因型个体的肌内脂肪和肌纤维密度显著高于GG型和GA型个体(P<0.05),而其他肉质性状无显著差异(P>0.05)。由此可见,对于肉质性状而言,AA是最有利基因型,本研究结果初步表明CAST基因G-198A多态位点的A等位基因是提高鸡肉质性状的一个潜在的DNA标记。Calpatatin（CAST） is the endogenous inhibitor of the calpain system, which has been found to affect the postmortem tenderization of skeletal muscle. This experiment was designed to study the effects of polymorphism of 5＇ flanking regulating of CAST gene on meat quality traits in broiler（Gallus gallus）, F、D、Y、B and S3 5 strains of quality broiler lines were used in the present study. The genetic polymorphisms of calpastatin（CAST） was investigated by polymerase chain reaction-restriction fragment length polymorphism（PCR- RELP）, and analyze the association of polymorphisms sites with quality broiler lines which included the PH value, Vitamin B1, muscle color, water holding capacity, intramuscular fat content and density of muscle fiber. In the present study, the 5＇ regulatory region of CAST gene of 300 broilers was amplified and a uniform fragment of 210 bp was obtained. The results indicated that one SNP site was detected in 5＇ flanking regulating of CAST gene. The 210 bp PCR product was digested with restriction endonuclease BMPI. Three genotypes（AA, AG and GG） were found at G- 198 A mutation locus at a distance of CDS- 198 locus in 300 broilers by digesting the PCR product with BMPI, and three frequency of AA, AG and GG genotypes was 0.3,0.2, 0.5, respectively in Y strain（0,125, 0.1, 0.775, respectively in S strain; 0.2, 0.375, 0.425, respectively in F strain; 0.05, 0.35, 0.6, respectively in D strain; 0.1897, 0.3966, 0.4138, respectively in B strain.）. The fitness test showed that all quality broiler lines at three mutation locus were in Hardy- Weinberg equilibrium. The relationship between CAST gene and meat quality traits in quality broiler was analyzed by least squares linear model. The individuals with AA genotype had significant effect on the intramuscular fat content and density of muscle fiber（P0.05）, and has no significant effect on the other meat quality traits（P0.05）. AA was the most favorable genotype for meat quality traits in broiler. The results preliminar

关 键 词：肉鸡 CAST基因 多态性 肉质性状 关联分析 

分 类 号：S831.2[农业科学—畜牧学]