Wolbachia对苹果蠹蛾生殖调控的作用  

The effects of Wolbachia on the reproduction of Cydia pomonella(L.)

作　　者：冯宏祖[1] 曹玉[1] 杨明禄[1] 刘慧敏[1] 许建军[2] 

机构地区：[1]塔里木大学/新疆建设兵团南疆特色果树生产工程实验室/农业部阿拉尔作物有害生物科学观测实验站/塔里木大学南疆农业有害生物综合治理重点实验室,新疆阿拉尔843300 [2]新疆农业科学院植物保护研究所,新疆乌鲁木齐830091

出　　处：《生物安全学报》2015年第4期306-309,共4页Journal of biosafety

基　　金：国家自然科学基金(31060242);新疆维吾尔自治区财政林业科技专项(新林计发[2014]40号文)

摘　　要：【背景】苹果蠹蛾是重要的果树害虫和检疫对象。研究显示,苹果蠹蛾体内所含的Wolbachia属于具有诱导胞质不亲和(CI)现象的A组中Dor亚组。本研究目的在于摸清苹果蠹蛾体内的Wolbachia对宿主的生殖调控是否具有诱导胞质不亲和的功能。【方法】通过饲喂抗生素消除苹果蠹蛾体内的Wolbachia,比较感染与消除Wolbachia的苹果蠹蛾生态适合度以及Wolbachia感染对苹果蠹蛾生殖的影响。【结果】消除Wolbachia后,苹果蠹蛾F_1、F_2代各虫态的发育历期、产卵量、孵化率和蛹的存活率与对照无显著差异;Wolbachia对苹果蠹蛾成虫的寿命、产卵前期、平均产卵量和性别比例均无影响。不同处理苹果蠹蛾之间的杂交试验结果表明,4种杂交的苹果蠹蛾都能产卵,且卵可以正常孵化。【结论与意义】在自然状态下苹果蠹蛾体内的Wolbachia不能诱导其宿主的胞质不亲和现象。[Background]Codling moth（ Cydia pomonella） is the most serious fruit tree and quarantine pest in Chinese orchards.The species is host to Wolbachia,Dor subgroup of the A group,which induce cytoplasmic incompatibility. The aim of this study is to find out if the Wolbachia induce cytoplasmic incompatibility to reproductive regulation in C. pomonella. [Method]The relative fitness of C. pomonella with active Wolbachia was compared to individuals in which Wolbachia was eliminate by antibiotics. [Result]We found no significant differences in development duration,fecundity,hatchability and pupal survival of the F_1,and F_2 generations between the two groups. Wolbachia infection had no effect on adult life span,the pre-oviposition period,fecundity and female ratio in C. pomonella. Hybridization tests showed that hybrids could lay eggs,and these eggs were normal. [Conclusion and significance]Wolbachia in C. pomonella did not induce host cytoplasmic incompatibility.

关 键 词：WOLBACHIA 苹果蠹蛾 生殖调控 胞质不亲和 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]