间歇低氧对小鼠肝肾组织MDA含量及SOD活力的影响  

The Effects of Intermittent Hypoxia on MDA Content and SOD Activity in Liver and Kidney of Mice

作　　者：李文娟[1] 李明华[1] 

机构地区：[1]河南省漯河市漯河医学高等专科学校,462002

出　　处：《中国继续医学教育》2016年第1期66-67,共2页China Continuing Medical Education

摘　　要：目的探讨间歇低氧对小鼠肝肾组织MDA及SOD活力的影响。方法将24只C57BL/6J雄性小鼠随机分为对照组和间歇低氧组,间歇低氧组置于自制的低氧舱中,舱内循环充入氮气和压缩空气,舱内氧浓度最高为(21±0.5)%、最低为(5±0.5)%,每次循环90 s,每天低氧8 h;对照组置于与间断低氧舱相似的舱内,舱内循环充入洁净压缩空气。12周后将小鼠处死,快速取出其肝肾组织并检测MDA的含量及SOD活力。结果肝组织中MDA含量的变化:低氧组比对照组高50.62%(P<0.01);肾组织中MDA含量的变化:低氧组比对照组高37.5%(P<0.01)。肝组织中SOD活力的变化:低氧组比对照组低14.44%(P<0.01);肾组织中SOD活力的变化:低氧组比对照组低22.21%(P<0.01)。结论间歇低氧小鼠体内存在着氧化应激状态。Objective To study the effects of intermittent hypoxic on sperm number and activity in mice. Methods twenty-four male C57BL/6J mice were randomly divided into two groups,namely the control group and intermittent hypoxic group. intermittent hypoxic group were housed in homemade low oxygen chambers which circle filled with N2 and compressed air（ the lower oxygen concentration is（5±0.5）% and the upper（21±0.5）%,90 s for each circle）8h each day. The control group was placed four similar cabins which circle filled with compressed air. 12 weeks later,the mice were sacrificed for determine the content of malondialdehyde（MDA） and the activity of superoxide dismutase（SOD） in liver and kidney. Results The changes of MDA content in liver and kidney tissue of intermittent hypoxi group were higher than control group by 50.62%（P 0.01） and 37.5%（P 0.01）,respectively. The liver tissue SOD activity of intermittent hypoxi group were 14.44%（P 0.01） lower than control group,Changes of the activity of SOD in renal tissue： intermittent hypoxi group were lower 22.21%（P〈0.01） than the control groups. Conclusion There is oxidative stress state in mice which exposed Intermittent hypoxia.

关 键 词：间歇低氧 睡眠呼吸暂停综合征 MDA SOD 氧化应激 

分 类 号：R332[医药卫生—人体生理学]